Anti-RIPK1/RIP Monoclonal Antibody for WB, IP, ELISA - Q13546

Core Product Specifications and Parameters

Biological Background: RIPK1 Function and Localization

• RIPK1, also known as RIP1 or cell death protein RIP, is a serine-threonine kinase that acts as a critical regulator of TNF-mediated apoptosis, necroptosis, and inflammatory signaling (PubMed:17703191, PubMed:24144979, PubMed:31827280). Related references: PMID:17703191, PMID:24144979, PMID:31827280
• It exhibits kinase activity-dependent functions in cell death and kinase-independent scaffold functions in cell survival, including activation of the canonical NF-kappa-B pathway (PubMed:11101870, PubMed:19524512, PubMed:19524513). Related references: PMID:11101870, PMID:19524512, PMID:19524513
• RIPK1 regulates assembly of two death-inducing complexes: complex IIa (RIPK1-FADD-CASP8) for apoptosis and complex IIb (RIPK1-RIPK3-MLKL) for necroptosis.
• It inhibits RIPK3-dependent necroptosis by recruiting CASP8 via FADD, which cleaves RIPK1, thus limiting TNF-induced necroptosis (PubMed:19524512, PubMed:19524513, PubMed:29440439). Related references: PMID:19524512, PMID:19524513, PMID:29440439
• RIPK1 is predominantly localized in the cytoplasm and at the cell membrane, consistent with its role in TNFR1 signal transduction.
• Key post-translational modifications include phosphorylation, ubiquitination (isopeptide bond), and proteolytic cleavage by CASP8, regulating its pro-death and pro-survival functions.
• It also promotes transcription of pro-inflammatory cytokines such as IL-6, linking cell death to immune responses (PubMed:31827280, PubMed:31827281). Related references: PMID:31827280, PMID:31827281

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 289-490 of human RIPK1, a region encompassing part of the kinase domain; this design may favor detection of both full-length and kinase-containing fragments.
• For Western blot, expect a band around 76 kDa. Consider including appropriate controls to account for cleavage products that can arise during apoptosis or necroptosis.
• In immunoprecipitation, validate antibody performance with known RIPK1 interactors such as TRADD or FADD under appropriate stimulation conditions (e.g., TNF treatment).
• ELISA experiments may be optimized for quantification of RIPK1 in cell lysates or tissue homogenates; cross-reactivity with other RIP family members should be assessed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined sourcing channel for high-quality IVD raw materials, including validated antibody pairs, monoclonal antibodies, and bulk ancillary reagents for RIPK1-related research. We support diagnostic manufacturers in developing assays for cell death and inflammatory diseases, from early-stage R&D to clinical supply. With expertise in rare-target material sourcing, CamelBio helps you overcome procurement challenges and accelerate your IVD project timelines.