Anti-RIOX2/MINA53 Rabbit Monoclonal Antibody for WB and ELISA - Q8IUF8

Core Product Specifications and Parameters

Biological Background: RIOX2/MINA53 Function and Localization

• RIOX2 (also known as MINA53, MDIG, NO52, JMJD10, ROX) is a ribosomal oxygenase that functions as both a histone lysine demethylase and a ribosomal histidine hydroxylase.
• It catalyzes the demethylation of trimethylated 'Lys-9' on histone H3 (H3K9me3), leading to increased ribosomal RNA expression, and hydroxylates 60S ribosomal protein L27a at His-39.
• RIOX2 plays important roles in cell growth and survival and is involved in ribosome biogenesis, likely during the assembly of pre-ribosomal particles.
• The protein contains a JmjC domain with dioxygenase activity, requiring iron as a cofactor, and is classified under oxidoreductases.
• Subcellular localization studies show RIOX2 is present in the nucleus, specifically enriched in the nucleolus, consistent with roles in rRNA transcription and ribosome assembly.
• RIOX2 is expressed in liver, skeletal muscle, heart, pancreas, and placenta, but not in brain, lung, or kidney; it is highly upregulated in various cancers including lung, esophageal, and colon tumors.
• The protein undergoes alternative splicing and is phosphorylated, with phosphoproteomics identification in reference proteome databases.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 350-450 of human RIOX2/MINA53; the antibody may recognize epitopes within this region. Consider verifying reactivity with truncated or mutant constructs in your assay system.
• For Western blot (WB), a band around 53 kDa is expected. Validate detection using whole-cell lysates from human, mouse, or rat cell lines known to express RIOX2. Include appropriate loading controls and consider using reducing conditions unless otherwise noted.
• For ELISA, direct or sandwich formats may be evaluated. Antigen coating with purified protein or peptide may be required. Optimize antibody concentration and detection conditions empirically.
• Given the upregulation in cancer, consider using cancer cell lines (e.g., lung, esophageal, colon) as positive controls and corresponding normal tissues as negative controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop IVD raw-material sourcing partner for diagnostic manufacturers, laboratories, and research institutes. We provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing for targets such as RIOX2/MINA53 in the areas of epigenetics, cancer biomarkers, and ribosome biology. Our integrated supply chain supports every stage from concept to clinic.