Anti-RIOK2 Rabbit Polyclonal Antibody for WB, ELISA - Q9BVS4

Core Product Specifications and Parameters

Biological Background: RIOK2 Function and Localization

• RIOK2 (RIO kinase 2) is a serine/threonine-protein kinase encoded by the RIOK2 gene.
• It plays a critical role in ribosome biogenesis by participating in the final cytoplasmic maturation steps of the 40S ribosomal subunit. Its kinase activity is essential for the release of factors NOB1, PNO1, and LTV1 from the late pre-40S particle and for processing 18S-E pre-rRNA to mature 18S rRNA. Related references: PMID:19564402
• RIOK2 also regulates the timing of the metaphase-anaphase transition during mitosis, and its phosphorylation, likely mediated by PLK1, controls this function. Related references: PMID:21880710
• The protein localizes predominantly in the cytoplasm where it exerts its functions.
• RIOK2 contains an ATP-binding domain and requires magnesium as a cofactor for its kinase activity. It undergoes phosphorylation, which modulates its activity in both ribosome biogenesis and cell cycle control.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fragment covering amino acids 253-552, encompassing the C-terminal portion of RIOK2. This region may include functional domains, and the antibody could recognize both native and denatured forms; validate in your specific assay system.
• For western blot (WB), suggested detection of a band at approximately 63 kDa in human samples; optimize loading and antibody dilution according to your experimental conditions.
• For ELISA, consider using the immunogen or recombinant full-length protein as coating antigen to assess binding; cross-species reactivity has not been tested but can be explored if needed.
• The antibody is shipped on ice bags and should be stored at the recommended temperature upon arrival to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and labs with reliable access to IVD-grade antibodies, including kinases like RIOK2, supporting research into ribosomal maturation and cell division. Our supply chain ensures validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline your assay development. Partner with us for rare-target sourcing and technical consultation from concept to clinic.