Anti-RING1B/RNF2 Monoclonal Antibody for WB, IP, and ELISA - Q99496

Core Product Specifications and Parameters

Biological Background: RING1B/RNF2 Function and Localization

• RING1B/RNF2, also known as RING2, RING1b, or BAP-1, is an E3 ubiquitin-protein ligase that catalyzes monoubiquitination of histone H2A at Lys-119 (H2AK119Ub), a key modification for epigenetic transcriptional repression and X chromosome inactivation. Related references: PMID:15386022 PMID:16359901 PMID:21772249
• It is an essential component of the Polycomb repressive complex 1 (PRC1), which maintains the silenced state of developmental genes, including Hox clusters, through chromatin modification. Related references: PMID:16359901 PMID:26151332
• The E3 ligase activity of RING1B/RNF2 is enhanced by the PRC1 subunit BMI1/PCGF4. Related references: PMID:21772249
• RNF2 localizes predominantly in the nucleus but can also be found in the cytoplasm and associated with chromosomes, reflecting its dynamic role in chromatin regulation.
• The protein contains a RING-type zinc finger domain and is subject to post-translational modifications including acetylation and phosphorylation, which may modulate its function.
• RING1B/RNF2 has been implicated in cell-cycle-dependent chromosomal association and is negatively regulated by AURKB in resting lymphocytes to maintain transcription.
• Additionally, it acts as a negative regulator of autophagy by ubiquitinating AMBRA1, targeting it for degradation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide within amino acids 100–200 of human RING1B/RNF2; this region is predicted to be surface-accessible based on structural models and should yield antibodies that recognize native protein conformations in immunoprecipitation (IP) and ELISA.
• For western blotting (WB), the predicted molecular weight is ~38 kDa; consider using appropriate protein ladder markers and verify band specificity in relevant human, mouse, and rat cell lysates.
• In IP experiments, pre-clearing lysates and optimizing antibody-to-protein ratios can help minimize background; cross-reactivity with mouse and rat orthologs allows for comparative studies.
• For ELISA, titrate the antibody using recombinant protein or peptide standards to determine optimal working concentrations; note that this antibody is monoclonal and may exhibit high specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive sourcing platform for IVD raw materials, offering validated antibody pairs, optimized monoclonals, and bulk ancillary reagents. For researchers focused on epigenetic regulation and Polycomb biology, this RING1B/RNF2 monoclonal antibody exemplifies our commitment to supplying quality components for assay development and diagnostic workflows. Our global supply chain enables rapid access to rare targets and bulk quantities, streamlining your path from research to clinical application.