Anti-RING1B/RNF2 Monoclonal Antibody for WB, IHC-P, ELISA - Q99496

Core Product Specifications and Parameters

Biological Background: RING1B/RNF2 Function and Localization

• RING1B/RNF2 (also known as RING2, RING1b, BAP-1, DinG) is an E3 ubiquitin-protein ligase that catalyzes the monoubiquitination of histone H2A at lysine 119 (H2AK119Ub), a key epigenetic mark associated with transcriptional repression.
  Related references:
  PMID:15386022
  PMID:16359901
  PMID:21772249

• It is an essential component of the Polycomb repressive complex 1 (PRC1), which maintains the silenced state of Hox genes and other developmental regulators through chromatin remodeling.
  Related references:
  PMID:16359901
  PMID:26151332

• The E3 ligase activity of RING1B/RNF2 is enhanced by its interaction with BMI1/PCGF4, and it acts as the primary catalytic subunit for H2A ubiquitination within PRC1.
  Related references:
  PMID:21772249

• RING1B/RNF2 localizes to the nucleus, cytoplasm, and chromosomes, with its chromosomal association being cell-cycle dependent.

• In resting B and T lymphocytes, RING1B/RNF2 interacts with AURKB (Aurora kinase B), which blocks its E3 ligase activity, thereby maintaining transcription.

• The protein contains a zinc-finger domain and is subject to post-translational modifications including acetylation and phosphorylation. Mutations in RNF2 are implicated in neurological disorders such as epilepsy and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering the full-length human RING1B/RNF2 (aa 1-336). This design may allow recognition of native protein conformations across multiple applications.
• For western blot (WB), a band around 38 kDa is expected; however, due to potential post-translational modifications or isoforms, validate the observed molecular weight in your specific sample system.
• The antibody is reported to cross-react with mouse and rat according to the supplier. Consider verifying cross-reactivity in your experimental model by comparing sequence homology.
• For immunohistochemistry (IHC-P), antigen retrieval conditions and detection protocols should be empirically optimized due to nuclear and cytoplasmic localization.
• Include appropriate positive and negative controls (e.g., cell lines with known RING1B expression) when performing WB or IHC-P.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and research teams with comprehensive access to IVD raw materials and technical services—from initial concept through clinical applications. For your epigenetic and oncology projects, CamelBio can supply validated antibody pairs, bulk quantities of monoclonal or polyclonal antibodies, and ancillary reagents. We also offer tailored sourcing for rare or custom targets, helping streamline your assay development pipeline.