Anti-Rig-I/DDX58 Rabbit Polyclonal Antibody for WB and ELISA - O95786

Core Product Specifications and Parameters

Biological Background: Rig-I/DDX58 Function and Localization

• RIG-I (DDX58) is an ATP-dependent RNA helicase and key innate immune receptor that detects cytoplasmic viral nucleic acids, triggering type I interferon and pro-inflammatory cytokine production. Related references: PMID:15208624, PMID:15708988, PMID:16125763
• The protein recognizes 5'-triphosphorylated double-stranded RNA (dsRNA) and short dsRNAs, with blunt-end base pairing at the 5'-end essential for activation, while overhangs modulate signaling. Related references: PMID:15208624, PMID:15708988, PMID:19576794
• Upon ligand binding, RIG-I oligomerizes into filaments and recruits the E3 ubiquitin ligase RNF135, leading to MAVS/IPS1 association and downstream activation of TBK1, IKBKE, and IRF3/7 transcription factors. Related references: PMID:28469175, PMID:31006531
• It detects a broad spectrum of RNA viruses including paramyxoviruses, rhabdoviruses, orthomyxoviruses, flaviviruses, rotaviruses, and reoviruses, and also senses dsDNA viruses like Epstein-Barr virus via RNA polymerase III transcripts. Related references: PMID:21616437, PMID:21884169, PMID:19631370
• Subcellular localization includes cytoplasm, ruffle membrane, cytoskeleton, and tight junctions, consistent with its role in intracellular viral surveillance.
• Post-translational modifications such as acetylation, phosphorylation, and ubiquitination regulate its activity, and it is subject to alternative splicing.
• Tissue specificity: Present in vascular smooth muscle cells (at protein level).
• Additional roles may include granulocyte production, bacterial phagocytosis, and cell migration regulation.

Experimental Guidance and Technical Tips

• For Western blot, consider using whole cell lysates from human or rat cells stimulated with viral mimetics (e.g., poly(I:C)) to enhance RIG-I expression; validate specificity with siRNA knockdown or knockout controls.
• The polyclonal nature may detect multiple bands; the primary band is expected at ~107 kDa. Confirm molecular weight using appropriate markers.
• In ELISA, the immunogen peptide (aa 825-925) can serve as a positive control for antibody binding; titrate the antibody to optimize signal-to-noise ratio.
• Cross-reactivity with rat is reported; for other species, validate in the relevant sample system before use.

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CamelBio provides diagnostic manufacturers and research labs with one-stop access to validated IVD raw materials, including this Rig-I/DDX58 rabbit polyclonal antibody. Our portfolio covers key innate immunity targets, supported by optimized antibody pairs, bulk ancillary reagents, and custom sourcing for rare or difficult-to-express proteins. Let our B2B supply chain accelerate your development from concept to clinic.