Anti-Rig-I/DDX58 Rabbit Monoclonal Antibody for WB, ELISA - O95786

Core Product Specifications and Parameters

Biological Background: Rig-I/DDX58 Function and Localization

• RIG-I is a cytoplasmic innate immune receptor that recognizes viral double-stranded RNA and 5'-triphosphorylated single-stranded RNA, triggering a signaling cascade that leads to type I interferon and pro-inflammatory cytokine production. Related references: PMID:15208624, PMID:15708988, PMID:16125763
• Upon RNA binding, RIG-I homooligomerizes into filaments and recruits the E3 ubiquitin ligase RNF135, which activates and amplifies antiviral signaling through ubiquitination-dependent and -independent mechanisms. Related references: PMID:28469175, PMID:31006531
• RIG-I detects a broad spectrum of RNA viruses, including influenza, measles, hepatitis C, dengue, and West Nile viruses, as well as SARS-CoV-2; it also senses some DNA viruses like Epstein-Barr virus via RNA polymerase III-transcribed RNAs. Related references: PMID:21616437, PMID:21884169, PMID:19631370
• Ligand specificity requires a 5'-triphosphate group and blunt-end base pairing at the 5'-end; 5'-overhangs abolish activity, while 3'-overhangs have minor effects. Related references: PMID:15208624, PMID:15708988, PMID:19576794
• Beyond viral sensing, RIG-I may participate in granulocyte differentiation, bacterial phagocytosis, and cell migration.
• RIG-I predominantly localizes to the cytoplasm, but also associates with cell projections, the cytoskeleton, and tight junctions; it is expressed in vascular smooth muscle cells.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is approximately 107 kDa. Use appropriate protein markers and consider loading controls to confirm band size.
• The immunogen corresponds to amino acids 241–455, spanning the helicase core domain. This region is conserved across human, mouse, and rat, consistent with the reported cross-reactivity; however, validation in target species is recommended.
• For ELISA, pair with a detection antibody targeting a non-overlapping epitope to avoid competition. Optimize coating and blocking conditions for best signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research laboratories with a wide range of monoclonal antibodies and ancillary reagents. For studies on innate immunity and viral infection, we offer validated antibody pairs, bulk quantities, and custom procurement of rare-target reagents to support your assay development and production needs.