Anti-Rig-I/DDX58 Monoclonal Antibody for WB, ELISA - O95786

Core Product Specifications and Parameters

Biological Background: Rig-I/DDX58 Function and Localization

• RIG-I (retinoic acid-inducible gene I), also known as DDX58, is an ATP-dependent RNA helicase belonging to the RIG-I-like receptor (RLR) family of innate immune sensors.
• Functions as a cytoplasmic innate immune receptor that detects viral nucleic acids and triggers signaling cascades leading to type I interferon and pro-inflammatory cytokine production. Related references: PMID:15208624, PMID:15708988, PMID:16125763
• Recognizes 5'-triphosphorylated ssRNA and dsRNA, with a critical requirement for blunt-end base pairing at the 5'-end; short dsRNAs (<1 kb) are effective ligands. Related references: PMID:15208624, PMID:15708988, PMID:19576794
• Upon ligand binding, RIG-I homooligomerizes and recruits the E3 ligase RNF135, leading to MAVS association, TBK1/IKBKE activation, and IRF3/IRF7 phosphorylation to induce antiviral genes. Related references: PMID:15208624, PMID:15708988, PMID:28469175, PMID:31006531
• Detects a broad range of positive- and negative-strand RNA viruses, including influenza, hepatitis C, dengue, and respiratory syncytial virus, as well as rotaviruses and reoviruses. Related references: PMID:21616437, PMID:21884169
• Also mediates antiviral responses to dsDNA viruses such as Epstein-Barr virus by recognizing dsRNA intermediates transcribed by RNA polymerase III. Related references: PMID:19631370
• Localizes to the cytoplasm, cytoskeleton, cell projections (ruffle membranes), and tight junctions; expressed in vascular smooth muscle cells.
• Undergoes acetylation and phosphorylation; harbors zinc finger and helicase domains; participates in host-virus interactions through ubiquitylation and isopeptide bond formation.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is approximately 107 kDa. Consider using positive controls such as virally infected human cell lines (e.g., A549 stimulated with poly(I:C) or infected with Sendai virus) to verify RIG-I induction, as basal expression may be low in unstimulated cells.
• Since the immunogen is a synthetic peptide, the antibody is expected to recognize a linear epitope. Validate detection of endogenous RIG-I in your sample system and optimize blocking and washing conditions to reduce nonspecific binding.
• In ELISA, the antibody can be employed as a capture or detection reagent. For quantitative assays, pairing with a matched detection antibody is recommended.
• Store as recommended on ice bag upon receipt and maintain at -20°C or -80°C for long-term storage; minimize freeze-thaw cycles.
• This antibody is raised against human RIG-I and validated for human samples; cross-reactivity with other species has not been determined and should be verified if used in non-human models.

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