Anti-RIC8A Rabbit Polyclonal Antibody for WB and ELISA - Q9NPQ8

Core Product Specifications and Parameters

Biological Background: RIC8A Function and Localization

• RIC8A (synembryn-A) is a chaperone that specifically binds and folds nascent G alpha proteins (GNAI1, GNAO1, GNA13, GNAQ) prior to heterotrimer formation, ensuring their stability and activity.
• It acts as a guanine nucleotide exchange factor (GEF), stimulating GDP/GTP exchange on G alpha subunits.
• RIC8A is involved in mitotic regulation by stimulating G(i)-alpha (GNAI1) to control microtubule pulling forces during chromosome movement.
• It also enhances G(q)-coupled receptor-mediated ERK activation, serving as an activator for GNAQ. Related references: PMID:16629901
• The protein localizes to the cytoplasm and cell cortex.
• Alternative splicing yields multiple isoforms.
• RIC8A is a phosphoprotein, suggesting post-translational regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 362-531 of human RIC8A, a region conserved in mouse and rat, supporting the observed cross-reactivity.
• For Western blotting, a specific band near 60 kDa is anticipated; validate the molecular weight in your sample system.
• Optimize antibody dilution and incubation conditions empirically for your specific experimental setup.
• For ELISA, the antibody may be used as a capture or detection reagent; confirm performance in your assay format.

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