Anti-RHOU Polyclonal Antibody for WB, ELISA - Q7L0Q8

Core Product Specifications and Parameters

Biological Background: RHOU Function and Localization

• RHOU, also known as WRCH1, CDC42L1, and ARHU, is a member of the Rho family of small GTPases that is ubiquitously expressed, with highest levels in stomach, small intestine, brain, skeletal muscle, and placenta.
• It binds to and activates protein kinase PAK1 (PubMed:11459829). Related references: PMID:11459829
• Plays a role in the regulation of cell morphology, cytoskeletal organization, and focal adhesion assembly during cell migration (PubMed:11459829, PubMed:17620058, PubMed:18086875). Related references: PMID:11459829, PMID:17620058, PMID:18086875
• Stimulates quiescent cells to reenter the cell cycle (PubMed:11459829). Related references: PMID:11459829
• Has no detectable GTPase activity but exhibits high intrinsic guanine nucleotide exchange activity, suggesting it is constitutively GTP-bound (PubMed:16472646). Related references: PMID:16472646
• Subcellular localization includes the cell membrane, Golgi apparatus membrane, cell junction, focal adhesion, and podosome.
• Undergoes multiple post-translational modifications including phosphorylation, palmitoylation, and methylation, and is subject to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 40–100 of human RHOU; consider verifying reactivity in your specific sample type, especially when using non-human systems.
• For Western blot detection, the predicted molecular weight of RHOU is ~28 kDa; choose an appropriate gel percentage and transfer conditions to resolve this small GTPase.
• Polyclonal antibodies may exhibit lot-to-lot variability; validate each new lot against a known positive control before use.
• In ELISA, include appropriate negative controls (e.g., pre-immune serum or an isotype control) to confirm signal specificity.
• Due to RHOU’s constitutive GTP-binding state, native conformation-sensitive applications may require optimization of lysis and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For research targets like RHOU involved in cell migration and cytoskeletal dynamics, we offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our sourcing capabilities extend to rare targets, enabling customized raw-material solutions for streamlined assay development.