Anti-RHOT1/MIRO1 Polyclonal Antibody for WB, ELISA - Q8IXI2

Core Product Specifications and Parameters

Biological Background: RHOT1/MIRO1 Function and Localization

• RHOT1 (also known as MIRO1, ARHT1) encodes mitochondrial Rho GTPase 1, an atypical mitochondrial nucleoside-triphosphatase (NTPase) critical for mitochondrial trafficking and subcellular distribution (PubMed:12482879, PubMed:16630562, PubMed:22396657). Related references: PMID:12482879 PMID:16630562 PMID:22396657
• The protein localizes to the mitochondrial outer membrane and is involved in anterograde mitochondrial transport, linking mitochondria to motor proteins via adaptor complexes.
• RHOT1/MIRO1 possesses GTPase activity and can hydrolyze GTP, ATP, and UTP, acting as a key regulator of mitochondrial dynamics (PubMed:30513825). Related references: PMID:30513825
• It promotes mitochondrial fission under high calcium conditions, acting as a calcium-responsive switch in mitochondrial shape changes (PubMed:27716788). Related references: PMID:27716788
• Belongs to the Rho family of small GTPases, but is classified as atypical due to its unique domain architecture and mitochondrial targeting.
• Ubiquitously expressed, with high levels in heart and skeletal muscle, reflecting its role in tissues with high energy demands.
• Post-translational modifications include acetylation and ubiquitin conjugation (isopeptide bond), which may modulate its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 310–590, a region likely spanning the GTPase domain and C-terminal tail; this epitope may be conserved across species, supporting the observed human, mouse, and rat reactivity.
• For Western blot, endogenous RHOT1/MIRO1 runs at approximately 71 kDa; consider loading a mitochondrial-enriched fraction to enhance signal. Validate antibody specificity with siRNA knockdown or knockout controls if available.
• In ELISA, use the recombinant immunogen as coating antigen or capture standard; cross-reactivity with related Rho GTPases should be evaluated due to the polyclonal nature.
• As this is a polyclonal antibody, lot-to-lot variation may occur; include appropriate positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and researchers with streamlined access to validated IVD raw materials, including this polyclonal antibody against the mitochondrial trafficking regulator RHOT1/MIRO1. Beyond individual antibodies, we supply matched antibody pairs, bulk monoclonal and polyclonal preparations, and ancillary reagents for assay development. For projects requiring customized sourcing of rare targets or large-volume production, our integrated supply chain can bridge the gap from concept to clinic.