Anti-RHOH Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q15669

Core Product Specifications and Parameters

Biological Background: RHOH Function and Localization

• RHOH (Rho-related GTP-binding protein RhoH), also known as TTF (Translocation three four protein), belongs to the Rho GTPase family but is atypical: it binds GTP yet lacks intrinsic GTPase activity and is resistant to RhoGAPs.
• It functions as a critical negative regulator of hematopoietic progenitor cell proliferation, survival, and migration; essential for thymocyte development and TCR signaling by mediating ZAP70 recruitment and activation.
• Required for CD3Z phosphorylation, ZAP70 membrane translocation, and downstream LAT signalosome activation during pre-TCR and TCR signaling, ensuring efficient beta-selection and positive selection.
• Plays key roles in mast cell function by facilitating SYK phosphorylation in FcεRI-mediated signal transduction and enabling calcium mobilization.
• Inhibits NF-κB and CRK/p38 activation by TNF, and negatively regulates leukotriene production in neutrophils.
• Subcellular localization: predominantly cytoplasmic and associated with the cell membrane.
• Tissue specificity: expression restricted to hematopoietic cells (highest in thymus, lower in spleen/bone marrow); enriched in TH1 T-helper cells; upregulated in neutrophils under inflammatory conditions (e.g., cystic fibrosis, colitis).

Experimental Guidance and Technical Tips

• The immunogen comprises the full-length human RHOH (amino acids 1-191), making this polyclonal antibody suitable for detecting multiple epitopes; optimal dilution and conditions should be determined empirically for each application.
• For Western blotting (WB), the predicted molecular weight is ~21 kDa; use a 12–15% SDS-PAGE gel and validate with hematopoietic cell lines (e.g., Jurkat, THP-1) known to express RHOH.
• For immunofluorescence/ICC, permeabilization is required for cytoplasmic/membrane staining; include appropriate controls given the restricted hematopoietic expression.
• Cross-reactivity with mouse is reported; this antibody may be used in mouse models, but validation in the relevant species and tissue is recommended.
• ELISA applications may be explored using recombinant protein as a standard; consider optimizing coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with reliable IVD raw materials, offering a comprehensive portfolio that includes validated antibody pairs, polyclonal antibodies like this anti-RHOH product, and bulk ancillary reagents. With expertise in hematopoietic and immune-related targets, CamelBio can streamline your procurement for rare or specialized antibodies and support the development of assays for T-cell signaling, mast cell function, or inflammatory disease research. Whether you need off-the-shelf antibodies or customized sourcing solutions, we serve as your strategic partner from concept to clinic.