Anti-RhoGDI Polyclonal Antibody for WB, ELISA - P52565

Core Product Specifications and Parameters

Biological Background: RhoGDI Function and Localization

• RhoGDI (Rho GDP-dissociation inhibitor 1), also known as Rho-GDI alpha, is encoded by the ARHGDIA gene and serves as a key regulator of Rho family GTPases.
• It specifically binds to Rho proteins such as CDC42, RAC1, and RHOA, inhibiting GDP dissociation and subsequent GTP binding, thereby maintaining them in an inactive state in the cytosol.
• RhoGDI facilitates the recycling and redistribution of activated Rho GTPases by extracting both inactive and activated prenylated forms from cellular membranes.
• Through its regulation of Rho proteins, RhoGDI modulates cell motility; in glioma cells, it suppresses migration and invasion via SEMA5A/PLXNB3-mediated inactivation of RAC1.
• The protein is predominantly localized in the cytoplasm.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, while alternative splicing generates multiple isoforms.
• Genetic variants of ARHGDIA are associated with disease, as indicated by the 'Disease variant' keyword in UniProt.

Experimental Guidance and Technical Tips

• The antibody is raised against recombinant full-length human RhoGDI (aa 1-204) and is validated for Western blot (WB) and ELISA.
• For WB, expect a band at approximately 23 kDa; use appropriate positive and negative controls, including knockout (KO) cell lysates if available, to confirm specificity.
• For ELISA, consider titrating the antibody and using recombinant RhoGDI as a standard to establish assay parameters.
• Cross-reactivity with mouse and rat has been reported, but verify performance in your specific model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to validated IVD raw materials. This anti-RhoGDI polyclonal antibody supports studies in Rho GTPase signaling and cell motility, and we can assist in sourcing complementary reagents such as matched antibody pairs, blocking buffers, or bulk quantities. Our expertise covers the full pipeline from concept to clinical application.