Anti-RhoGAP Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q13017

Core Product Specifications and Parameters

Biological Background: RhoGAP (p190-B) Function and Localization

• Rho GTPase-activating protein 5 (RhoGAP, also known as p190-B, GFI2) is encoded by the ARHGAP5 gene and belongs to the RhoGAP family.
• It functions as a GTPase-activating protein (GAP) for Rho family small GTPases, accelerating their intrinsic GTP hydrolysis activity. Related references: PMID:8537347
• Subcellular localization includes cytoplasm and cell membrane, consistent with its role in regulating Rho-mediated cytoskeletal dynamics and signal transduction.
• At the protein level, RhoGAP has been detected in human skin fibroblasts. Related references: PMID:8537347
• The protein undergoes post‑translational modifications such as nitration and phosphorylation, and alternative splicing produces multiple isoforms.
• It contains repeat regions and has been identified in proteomics studies.
• The molecular weight is approximately 172 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1292–1501 of human RhoGAP (NP_001164.2), a region likely specific to the p190‑B isoform. Validate specificity in your sample system.
• For western blot, use appropriate loading controls and confirm the expected band near 172 kDa.
• In immunofluorescence/ICC, include known positive (e.g., skin fibroblasts) and negative controls to confirm signal specificity.
• Cross‑reactivity with mouse and rat orthologs is reported; confirm reactivity in the species of interest before large‑scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with integrated access to high‑quality IVD raw materials, including validated antibody pairs and bulk ancillary reagents. For cell signaling and cytoskeleton studies, we offer optimized monoclonal and polyclonal antibodies targeting key regulators such as RhoGAP, supporting your workflow from concept to clinic. Our sourcing network can also accommodate requests for rare‑target raw materials.