Anti-RhoGAP (p190-B) Rabbit Monoclonal Antibody for WB, IP, ELISA - Q13017

Core Product Specifications and Parameters

Biological Background: RhoGAP Function and Localization

• Rho GTPase-activating protein 5 (ARHGAP5, also known as p190-B, RhoGAP5) is a member of the RhoGAP family that functions as a GTPase-activating protein (GAP) for Rho family small GTPases, enhancing their intrinsic GTP hydrolysis activity to terminate signaling (PubMed:8537347). Related references: PMID:8537347
• The protein undergoes alternative splicing, generating multiple isoforms that may exhibit tissue-specific expression and functional diversity.
• ARHGAP5 is primarily localized to the cytoplasm and can associate with the cell membrane, consistent with its role in regulating Rho GTPases at membrane-proximal signaling sites.
• The protein is a phosphoprotein, and its activity may be modulated by tyrosine phosphorylation, linking it to growth factor and integrin signaling pathways.
• It is specifically detected in skin fibroblasts at the protein level, suggesting a role in fibroblast biology and possibly in wound healing or connective tissue remodeling.
• Keywords from UniProt: 3D-structure, Alternative splicing, Cell membrane, Cytoplasm, GTPase activation, Membrane, Nitration, Phosphoprotein, Proteomics identification, Reference proteome, Repeat. These highlight its structural and regulatory features.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1150–1300 of human RhoGAP (Q13017), a region that may be well-conserved across species (cross-reacts with mouse and rat). Validate specificity in the relevant species by sequence alignment or initial Western blot analysis.
• For Western blotting, the predicted molecular weight is ~172 kDa. Use appropriate gel percentages (e.g., 6–8% SDS-PAGE) and loading controls. Confirm band specificity using positive control lysates (e.g., skin fibroblast lysates, based on tissue expression data).
• In immunoprecipitation (IP) experiments, optimize antibody concentration by titration and include appropriate IgG controls. Consider cross-linking the antibody to beads to reduce heavy chain interference in subsequent WB detection.
• For ELISA, pair with a matched detection antibody or use as a capture antibody depending on the assay format. Validate with recombinant RhoGAP protein or cell lysates expressing the target.
• Since the antibody is monoclonal (rabbit), expect consistent lot-to-lot performance. However, re-validate each new lot in your specific application and sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material provider, offering a wide selection of validated antibodies, including this RhoGAP monoclonal antibody, ideal for researchers studying Rho GTPase signaling pathways in cancer, cell migration, and cytoskeletal dynamics. In addition to high-quality primary antibodies, we supply ancillary reagents, bulk quantities, and custom sourcing services to support diagnostic assay development from concept to clinic. Let CamelBio be your reliable partner for all your IVD raw-material needs.