Anti-RHOG Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P84095

Core Product Specifications and Parameters

Biological Background: RHOG Function and Localization

• RHOG, also known as ARHG, is a Rho-related GTP-binding protein belonging to the Rho family of small GTPases.
• RHOG plays a critical role in immunological synapse formation by regulating F-actin density and architecture, modulating actin reorganization in lymphocytes possibly through Rac1. Related references: PMID:33513601
• It functions as an activator of the ELMO-DOCK-Rac pathway, essential for cell migration and cytoskeletal dynamics. Related references: PMID:32651375, PMID:38857861
• RHOG is required for the formation of membrane ruffles during macropinocytosis. Related references: PMID:15133129
• It mediates cell migration and the formation of cup-like structures during trans-endothelial migration of leukocytes. Related references: PMID:17875742
• In an activation-dependent manner, RHOG binds phospholipids, thereby anchoring proteins to the plasma membrane and facilitating exocytosis of cytotoxic granules by lymphocytes. Related references: PMID:33513601
• During Salmonella infection, RHOG is activated by SopB and ARHGEF26/SGEF, inducing cytoskeleton rearrangements that promote bacterial entry.
• RHOG localizes primarily to the cell membrane and is subject to multiple post-translational modifications, including prenylation, phosphorylation, and glycoprotein addition, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from the central region (aa 50-150) of human RHOG. As a polyclonal antibody, it may recognize multiple epitopes; validate specificity in your assay system using appropriate controls (e.g., antigen blocking, siRNA knockdown).
• For Western blot (WB), the antibody is expected to detect a band at approximately 21 kDa. Optimize loading amounts and detection conditions for your cell or tissue lysates.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), fixation and permeabilization conditions should be empirically determined. The antibody's cross-reactivity with mouse and rat samples can be advantageous for comparative studies.
• ELISA can be employed for quantitative detection; consider a direct or sandwich format based on your specific needs.
• Given RHOG's involvement in immunological synapse and exocytosis, this antibody may be useful in functional assays; however, confirm neutralizing activity if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing bridge for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For research on Rho GTPase signaling, immunological synapses, and cell migration, our supply chain ensures timely access to rare-target reagents and technical support. We connect diagnostic manufacturers and laboratories with the raw materials needed to advance from concept to clinic.