Anti-RHOD Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O00212

Core Product Specifications and Parameters

Biological Background: RHOD Function and Localization

• Rho-related GTP-binding protein RhoD (also known as RhoHP1) is a member of the Rho family of small GTPases, serving as a molecular switch in signal transduction cascades.
• RHOD is involved in endosome dynamics and coordinates membrane transport with cytoskeletal function, facilitating the internalization and trafficking of activated tyrosine kinase receptors such as PDGFRB.
• Through interaction with WHAMM, RHOD regulates reorganization of the actin cytoskeleton, promoting filopodia formation and actin filament bundling.
• RHOD can modulate the effect of DAPK3 in actin cytoskeleton reorganization and focal adhesion dissolution, influencing cell adhesion and motility.
• The protein localizes predominantly to the cell membrane and early endosomes, consistent with its role in endosomal trafficking and membrane-cytoskeleton coupling.
• Tissue expression is highest in heart, placenta, liver, skeletal muscle, and pancreas, with weaker expression in several other tissues.
• Key structural and functional features include GTP binding, prenylation, and association with cellular membranes, as reflected by keywords such as hydrolase, lipoprotein, and nucleotide-binding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 101–200 of human RHOD (NP_055393.1). Consider verifying antibody specificity against the target protein in relevant cell or tissue lysates.
• For Western blotting, RHOD migrates at approximately 23 kDa. Use positive control lysates from heart, liver, or skeletal muscle tissues known to express RHOD.
• In IHC-P and IF/ICC, staining is expected at the cell membrane and early endosomal compartments; validate localization patterns in your model system.
• For ELISA, the synthetic peptide immunogen can serve as a positive control to confirm antibody reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive, one-stop sourcing solution for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. This anti-RHOD polyclonal antibody aids in studies of Rho GTPase signaling, membrane trafficking, and cytoskeletal dynamics. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to meet your development needs.