Anti-RhoC Rabbit Polyclonal Antibody (KO Validated) for WB, ELISA - P08134

Core Product Specifications and Parameters

Biological Background: RhoC Function and Localization

• RhoC is a Rho-related GTP-binding protein, also known by aliases H9, ARH9, ARHC, RHOH9, and oC. It belongs to the Rho family of small GTPases.
• Serves as a molecular switch by cycling between an inactive GDP-bound and an active GTP-bound state, regulating downstream effectors involved in cytoskeletal dynamics.
• Regulates signal transduction pathways linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers, thereby influencing cell morphology and migration.
• Functions as a microtubule-dependent signal required for myosin contractile ring formation during cytokinesis, ensuring proper cell division.
• Regulates apical junction formation in bronchial epithelial cells, contributing to the maintenance of epithelial barrier integrity.
• Subcellular localization: Predominantly associated with the cell membrane and enriched at the cleavage furrow during mitosis, consistent with its roles in membrane receptor signaling and cytokinesis.
• Post-translational modifications include prenylation at the C-terminal CAAX motif for membrane anchorage, ADP-ribosylation, methylation, and potential glycosylation, all modulating its activity and localization.
• Implicated in key cellular processes such as cytoskeleton organization, cell proliferation, and migration; aberrant RhoC activity has been linked to cancer invasion and metastasis.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1-193 of human RhoC, which may elicit antibodies recognizing both GTP- and GDP-bound conformations.
• For Western blotting, anticipate a specific band at approximately 22 kDa; consider using RhoC-positive cell lines (e.g., HeLa or A549) as positive controls.
• Since the antibody is KO validated, confirming specificity in knockout versus wild-type samples is recommended for quantitative assays.
• For ELISA, evaluate direct coating with the immunogen or implement a sandwich format with a matched detection antibody; cross-reactivity with rat RhoC should be verified empirically.
• Given the high sequence homology among Rho family members, test for potential cross-reactivity with RhoA and RhoB in the user’s experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated sourcing partner for IVD raw materials, connecting diagnostic developers and research laboratories with validated antibodies like this anti-RhoC polyclonal antibody for signal transduction and cancer biology studies. We offer an extensive portfolio including optimized monoclonal/polyclonal antibodies, recombinant proteins, and ancillary reagents, all designed to streamline your assay development. Our capabilities also cover rare target sourcing and custom reagent services, ensuring you have the resources needed from early-stage research through clinical validation.