Anti-RhoC Polyclonal Antibody for WB, IF/ICC, ELISA - P08134

Core Product Specifications and Parameters

Biological Background: RhoC Function and Localization

• RhoC (Rho-related GTP-binding protein RhoC) is a small GTPase of the Rho family, encoded by the RHOC gene (also known as ARH9 and ARHC), and is closely related to RhoA and RhoB.
• Acts as a molecular switch in signal transduction, cycling between an inactive GDP-bound and active GTP-bound state, regulating actin cytoskeleton dynamics.
• Mediates the formation of focal adhesions and actin stress fibers downstream of plasma membrane receptors, essential for cell adhesion and migration.
• Plays a critical role in cytokinesis by serving as a microtubule-dependent signal required for myosin contractile ring formation at the cleavage furrow.
• Regulates apical junction formation in bronchial epithelial cells, contributing to epithelial barrier integrity.
• Localizes predominantly to the cell membrane and is enriched at the cleavage furrow during cell division.
• Post-translational modifications include prenylation, methylation, and ADP-ribosylation, which modulate its membrane association and activity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 94-193 of human RhoC (NP_786886.1). This region is likely to be unique, offering potential specificity for RhoC over other Rho family members; however, cross-reactivity should be verified in the relevant experimental system.
• For Western blot (WB), the antibody detects a band around 22 kDa in human, mouse, and rat samples. Optimize protein loading and antibody dilution based on target abundance in your samples.
• For immunofluorescence (IF/ICC), ensure proper fixation and permeabilization protocols to visualize membrane and cleavage furrow localization. Co-staining with cytoskeletal markers may aid interpretation.
• For ELISA, the antibody can be used as a capture or detection reagent; pair with a suitable secondary antibody and validate the detection range using recombinant RhoC protein.

CamelBio: Your One-Stop Sourcing Bridge

As a trusted one-stop supplier for IVD raw materials, CamelBio offers high-quality antibodies like this anti-RhoC polyclonal antibody to support your signal transduction and cell biology research. Beyond individual antibodies, we provide validated antibody pairs, bulk reagents, and customized sourcing solutions to accelerate your assay development from concept to clinic. Contact us to discuss your specific project requirements and explore our comprehensive portfolio.