Anti-RHOBTB2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BYZ6

Core Product Specifications and Parameters

Biological Background: RHOBTB2 Function and Localization

• RHOBTB2, also known as DBC2 (Deleted in breast cancer 2 gene protein) or p83, is a Rho-related BTB domain-containing protein 2 encoded by the RHOBTB2 gene.
• It functions as a regulator of cell proliferation and apoptosis. Related references: PMID:21801820
• RHOBTB2 acts as a substrate adapter that recruits key substrates, such as MSI2, to CUL3-based ubiquitin ligase complexes for ubiquitination and subsequent degradation. Related references: PMID:15107402, PMID:27941885
• The protein is required for MSI2 ubiquitination and degradation, thereby influencing the maintenance of hematopoietic stem cells and cancer cell growth. Related references: PMID:27941885
• RHOBTB2 is ubiquitously expressed, with highest levels in neural tissues; it is also detected in fetal lung, heart, and brain, suggesting diverse physiological roles.
• According to UniProt annotations, it is associated with alternative splicing, disease variants linked to epilepsy, GTP-binding, hydrolase activity, metal binding, and the ubiquitin conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 320–480, a region that may be exposed and accessible in native conformations, suitable for detecting endogenous RHOBTB2.
• Due to high sequence homology of the immunogen region among human, mouse, and rat, this antibody may recognize orthologs in these species; confirm cross-reactivity in your experimental system.
• For Western blotting (WB), expect a band at approximately 83 kDa; consider loading adequate protein amounts and using appropriate positive controls.
• For immunofluorescence/immunocytochemistry (IF/ICC), optimize fixation and permeabilization conditions to preserve epitope reactivity.
• For ELISA applications, consider using the recombinant immunogen as a positive control to optimize coating concentrations and antibody dilutions.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw‑material partner, CamelBio streamlines your development of RHOBTB2‑based assays for oncology and neurology research. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Count on CamelBio to bridge your concept‑to‑clinic journey with reliable supply solutions.