Anti-RhoB Rabbit Monoclonal Antibody for WB and ELISA - P62745

Core Product Specifications and Parameters

Biological Background: RhoB Function and Localization

• RhoB is a small GTP-binding protein of the Rho family, cycling between GDP-bound inactive and GTP-bound active states to regulate downstream signaling.
• It plays a critical role in mediating apoptosis in neoplastically transformed cells following DNA damage and is essential for genotoxic stress-induced cell death in breast cancer cells.
• RhoB acts as a tumor suppressor; its genetic deletion leads to spontaneous tumor formation, highlighting its negative regulatory role in tumorigenesis.
• It is involved in intracellular protein trafficking, targeting PKN1 to endosomes, directing the EGF receptor from late endosomes to lysosomes, and stabilizing AKT1 to promote endothelial cell survival.
• During cytokinesis, RhoB serves as a microtubule-dependent signal essential for the formation of the myosin contractile ring.
• Subcellularly, RhoB localizes to late endosome membranes, the cell membrane, the nucleus, and the cleavage furrow, consistent with its diverse functions.
• Post-translational modifications, including prenylation and palmitoylation, are crucial for its membrane association and functional localization.

Experimental Guidance and Technical Tips

• For Western blotting, a band around 22 kDa is expected for RhoB. Optimize lysate preparation and blocking conditions to minimize background.
• The immunogen is a synthetic peptide from the internal region (amino acids 70–150), suggesting the antibody likely recognizes total RhoB. For studies requiring isoform specificity, validate with appropriate controls.
• The antibody is suitable for ELISA; consider pairing with a detection antibody for sandwich ELISA development.
• Cross-reactivity is indicated for human, mouse, and rat. When using in other species, verify sequence homology and perform a pilot experiment.
• As a rabbit monoclonal antibody, it offers high batch-to-batch consistency. For critical experiments, titrate the antibody to determine the optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material sourcing, supporting diagnostic manufacturers, labs, and research institutes from concept to clinic. This anti-RhoB antibody exemplifies our commitment to providing high-quality immunological reagents for apoptosis, cancer, and cell biology research. Beyond single antibodies, CamelBio offers validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to streamline your assay development and production.