Anti-RhoB Polyclonal Antibody for WB, ELISA - P62745

Core Product Specifications and Parameters

Biological Background: RhoB Function and Localization

• RhoB is a Rho-related GTP-binding protein that mediates apoptosis in neoplastically transformed cells after DNA damage, and is required for genotoxic stress-induced cell death in breast cancer cells.
• It plays a negative role in tumorigenesis; deletion of RhoB promotes tumor formation, highlighting its function as a tumor suppressor.
• RhoB is involved in intracellular protein trafficking, including targeting PKN1 to endosomes and regulating the trafficking of the EGF receptor from late endosomes to lysosomes.
• It is required for the stability and nuclear trafficking of AKT1/AKT, thereby promoting endothelial cell survival during vascular development.
• RhoB serves as a microtubule-dependent signal necessary for myosin contractile ring formation during cell cycle cytokinesis.
• The protein localizes to multiple sites including the late endosome membrane, cell membrane, nucleus, and cleavage furrow.
• Post-translational modifications of RhoB include prenylation, palmitoylation, phosphorylation, and methylation, which regulate its subcellular localization and function.
• It is involved in key biological processes such as angiogenesis, cell adhesion, differentiation, and apoptosis, with its activity modulated by GTP binding and hydrolysis.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 100–190 of human RhoB; consider using peptide competition assays to confirm antibody specificity.
• For Western blotting, a band of approximately 22 kDa is expected. Validate detection in relevant positive and negative control samples.
• In ELISA applications, optimize coating antigen concentration and antibody dilution to achieve optimal signal-to-noise ratios.
• The antibody cross-reacts with mouse and rat RhoB, likely due to high sequence conservation; verify performance in your specific experimental system.
• Pre-adsorption with the immunizing peptide may be useful to control for non-specific binding in complex samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing solution for IVD raw materials, connecting diagnostic developers and researchers with validated antibody pairs, optimized polyclonal antibodies like this anti-RhoB reagent, and bulk ancillary supplies. Whether you are studying Rho GTPase signaling, apoptosis, or cancer biology, we streamline access to rare target raw materials and provide technical support to accelerate your development from concept to clinic.