Anti-RHOA/RHOB/RHOC Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q16695 / P68431

Core Product Specifications and Parameters

Biological Background: Histone H3.1t Function and Localization

• Histone H3.1t, also known as H3/g or Histone H3.4, is encoded by the H3-4 (H3FT, HIST3H3) gene and belongs to the histone H3 family.
• Serves as a core component of the nucleosome, wrapping and compacting DNA into chromatin to regulate DNA accessibility.
• Plays a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
• DNA accessibility is modulated by a complex set of post-translational modifications, collectively termed the histone code, which includes acetylation, methylation, phosphorylation, and ubiquitination.
• Localizes to the nucleus and is associated with chromosomes.
• Tissue specificity: Predominantly expressed in testicular cells.
• Post-translational modifications include acetylation, ADP-ribosylation, citrullination, hydroxylation, methylation, phosphorylation, and ubiquitination, impacting chromatin structure and function.
• Contains a histone fold domain that facilitates DNA binding and nucleosome assembly.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant fusion protein spanning amino acids 1-193 of human RHOA/RHOB/RHOC, suggesting a conserved epitope region.
• For western blotting (WB), a band at approximately 22 kDa is expected based on the detected protein weight.
• Suitable for immunohistochemistry (IHC-P), immunofluorescence (IF/ICC), and ELISA applications; consider optimizing antibody dilution for each specific sample and protocol.
• Validate cross-reactivity in human, mouse, and rat tissues or cell lines to confirm consistency across species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop platform for IVD raw-material procurement, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This RHOA/RHOB/RHOC Rabbit mAb supports advanced research in cell signaling and cytoskeletal dynamics. For complete IVD development support from concept to clinic, including technical services and consulting, partner with CamelBio.