Anti-RhoA Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P61586

Core Product Specifications and Parameters

Biological Background: RhoA Function and Localization

• Small GTPase of the Rho family, encoded by the RHOA gene (aliases: ARHA, ARH12, RHO12). Cycles between active GTP-bound and inactive GDP-bound states.
• Key regulator of actin cytoskeleton organization, focal adhesion assembly, and stress fiber formation, linking plasma membrane receptors to cytoskeletal dynamics. Related references: PMID:8910519 PMID:9121475
• Mediates cell migration, adhesion turnover, and cell cycle progression, including cytokinesis and cleavage furrow formation. Related references: PMID:12900402 PMID:16236794 PMID:19934221 PMID:20974804 PMID:23940119 PMID:23871831
• Participates in microtubule-dependent signaling: stabilizes microtubules at the cell cortex via the MEMO1-RHOA-DIAPH1 pathway and regulates APC/CLASP2 localization. Related references: PMID:20937854
• Modulates ion channel activity by promoting KCNA2 potassium channel endocytosis upon CHRM1 activation. Related references: PMID:9635436 PMID:19403695
• Functions as an allosteric activator of the guanine nucleotide exchange factor ECT2 and may activate phospholipase C epsilon (PLCE1). Related references: PMID:31888991 PMID:16103226
• Subcellular localization: cell membrane, cytoplasm, cytoskeleton, cleavage furrow, midbody, lamellipodium, dendrites, and nucleus.
• Post-translational modifications: prenylation, phosphorylation, methylation, ADP-ribosylation, and ubiquitination. Also subject to proteolytic cleavage by Yersinia pestis yopT during infection.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 94–193 of human RhoA. The antibody is expected to recognize full-length endogenous RhoA, with cross-reactivity predicted in human, mouse, and rat.
• For Western blot (WB), expect a specific band around 22 kDa. Validate detection conditions using appropriate positive and negative controls.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider antigen retrieval techniques and optimize antibody dilution based on your tissue or cell model.
• ELISA applications may require pairing with a matched detection reagent; consider titrating the antibody to determine the optimal coating and detection concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive B2B sourcing solutions for IVD raw materials, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target procurement to diagnostic manufacturers and research laboratories. This anti-RhoA polyclonal antibody supports studies in cytoskeletal dynamics, cell migration, and signaling pathways relevant to oncology, cardiovascular diseases, and neurological disorders. Partner with us to streamline your raw material pipeline from concept to clinic with reliable supply and technical expertise.