Anti-RhoA Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P61586

Core Product Specifications and Parameters

Biological Background: RhoA Function and Localization

• RhoA is a small GTPase that cycles between an active GTP-bound and an inactive GDP-bound state, primarily regulating cytoskeletal dynamics and cell migration. Related references: PMID:23871831
• It transduces signals from plasma membrane receptors to promote focal adhesion assembly and actin stress fiber formation. Related references: PMID:31570889, PMID:8910519, PMID:9121475
• RhoA is essential for cytokinesis, driving myosin contractile ring formation and cleavage furrow progression. Related references: PMID:12900402, PMID:16236794
• It plays a critical role in keratinocyte cell-cell adhesion by regulating apical junction formation. Related references: PMID:20974804, PMID:23940119
• RhoA controls cell migration and adhesion dynamics through the SPATA13-mediated pathway. Related references: PMID:19934221
• In the cell cortex, the MEMO1-RHOA-DIAPH1 signaling axis stabilizes microtubules and governs APC/CLASP2 localization. Related references: PMID:20937854
• It regulates potassium channel KCNA2 activity by promoting its endocytosis from the cell surface. Related references: PMID:19403695, PMID:9635436
• Subcellularly, RhoA localizes to the cell membrane, cytoplasm, cytoskeleton, cleavage furrow, midbody, lamellipodia, dendrites, and nucleus.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved central region (aa 94-193) of human RhoA, rationalizing the observed cross-reactivity with mouse and rat.
• For Western blotting, the predicted molecular weight is ~22 kDa; include appropriate positive control lysates (e.g., HeLa, NIH/3T3) and validate antibody specificity in your target tissue or cell line.
• When performing IHC, consider antigen retrieval methods and optimize fixation conditions to preserve epitope integrity in paraffin-embedded sections.
• For ELISA, titrate the antibody as both capture and detection reagent to determine optimal working concentrations for your specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw materials, offering a complete portfolio of validated antibodies, antigens, and ancillary reagents. This anti-RhoA polyclonal antibody exemplifies our ability to deliver high-quality tools for cytoskeleton, cell migration, and signal transduction research. Whether you need optimized monoclonal antibody pairs, bulk orders, or rare-target sourcing, CamelBio bridges your development needs from concept to clinic.