Anti-RHBDD1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q8TEB9

Core Product Specifications and Parameters

Biological Background: RHBDD1 Function and Localization

• RHBDD1 (also known as RRP4, RHBDL4) is a member of the rhomboid family of intramembrane serine proteases that cleave transmembrane substrates within the lipid bilayer, releasing functional domains (UniProt Q8TEB9).
• It plays a critical role in endoplasmic reticulum-associated degradation (ERAD) by mediating the transfer of specific misfolded membrane proteins from the ER to the cytosol for ubiquitin-dependent proteasomal degradation.
• RHBDD1 processes multiple substrates including BIK, MPZ, PKD1, PTCRA, RHO, STEAP3, and TRAC, thereby regulating diverse processes from apoptosis to cellular differentiation.
• The protease modulates apoptotic signaling by controlling the activity of BIK, a pro-apoptotic BCL-2 family member, and inhibits apoptosis in spermatogonia to support spermatogenesis.
• It localizes to both the endoplasmic reticulum membrane and the mitochondrion membrane, consistent with its roles in ER quality control and apoptosis regulation.
• RHBDD1 is highly expressed in the testis, where its anti-apoptotic function is essential for germ cell survival and normal spermatogenesis.
• The protein contains multiple transmembrane helices and features a serine protease catalytic domain; alternative splicing generates different isoforms (UniProt Q8TEB9 Keywords: Alternative splicing, Serine protease, Transmembrane helix).
• Additionally, RHBDD1 is involved in exosomal secretion pathways, inhibiting the TSAP6-mediated route and thereby influencing intercellular communication.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 206–315 of human RHBDD1, which lies within a region likely to be immunogenic; for Western blot, expect a band around 36 kDa, though post-translational modifications may alter migration.
• For immunofluorescence/ICC, optimize permeabilization and fixation conditions due to the transmembrane nature of RHBDD1; co-staining with ER or mitochondrial markers can confirm localization.
• Consider validating antibody specificity using knockout cell lines or siRNA-mediated knockdown in relevant samples (e.g., testis-derived cells) for definitive identification.
• The antibody’s cross-reactivity with mouse and rat allows for comparative studies; however, always verify performance in the specific species and application context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with comprehensive access to IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For scientists studying intramembrane proteolysis, ER-associated degradation, or apoptosis, CamelBio can source rare-target raw materials like RHBDD1 antibodies and assay components. Partner with us to streamline your procurement from concept to clinic with reliable, scalable solutions.