Anti-RGS7 Polyclonal Antibody for WB and ELISA - P49802

Core Product Specifications and Parameters

Biological Background: RGS7 Function and Localization

• RGS7 acts as a GTPase-activating protein (GAP) component of the RGS7-GNB5 complex, accelerating GTP hydrolysis on G protein alpha subunits such as GNAO1 to terminate GPCR signaling. Related references: PMID:10521509 PMID:10862767

• The RGS7-GNB5 complex inhibits signal transduction by promoting the inactive GDP-bound state of G protein alpha subunits, thereby regulating downstream effector pathways. Related references: PMID:10521509 PMID:10862767 PMID:31189666

• Modulates the activity of potassium channels activated by GNAO1 in response to muscarinic acetylcholine receptor M2/CHRM2 signaling, influencing neuronal excitability. Related references: PMID:15897264

• May play a role in synaptic vesicle exocytosis, potentially through its GTPase-regulating function (Probable). Related references: PMID:12659861

• Localizes to the cytoplasm, cytosol, and cell membrane, consistent with its role in membrane-proximal signaling events.

• Post-translational modifications include palmitoylation and phosphorylation, and alternative splicing generates multiple isoforms, contributing to functional diversity.

Experimental Guidance and Technical Tips

• For Western blot analysis, consider using whole-cell lysates from human, mouse, or rat tissues and validate the detection of a band near 58 kDa corresponding to RGS7.
• The immunogen spans residues 1-300 (N-terminal region); for experiments targeting C-terminal regions or specific isoforms, verify sequence coverage.
• For ELISA, titrate the antibody to determine optimal coating or detection concentrations, and include appropriate negative controls to confirm specificity.
• Due to the polyclonal nature, each new lot should be validated for consistency in your specific assay conditions.
• Cross-reactivity with other RGS family members has not been tested; validate specificity using recombinant proteins or knockout controls if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop sourcing solution for IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents. This anti-RGS7 polyclonal antibody supports studies in GPCR signaling and neurological research, and we can assist with bulk procurement, rare-target sourcing, and custom development. We simplify the journey from concept to clinic, ensuring reliable supply for your diagnostic and research applications.