Anti-RGS5 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O15539

Core Product Specifications and Parameters

Biological Background: RGS5 Function and Localization

• RGS5 (Regulator of G protein signaling 5) is a member of the RGS protein family, which negatively regulates G protein-coupled receptor (GPCR) signaling pathways.
• It functions as a GTPase-activating protein (GAP) for G protein alpha subunits, specifically enhancing GTP hydrolysis on G(i)-alpha and G(o)-alpha, thereby promoting their inactive GDP-bound state and inhibiting signal transduction.
• RGS5 does not bind to or regulate G(s)-alpha subunits, indicating selectivity in its inhibitory action.
• The protein is localized to the cytoplasm and membrane, consistent with its role in modulating membrane-associated G protein signaling.
• RGS5 is documented to undergo alternative splicing, and its structure has been determined by 3D-structure studies.
• Proteomics identification supports its expression and reference proteome status, confirming its relevance in human biology.

Experimental Guidance and Technical Tips

• Since the antibody is raised against full-length human RGS5 (aa 1-181), it may recognize multiple isoforms if they share the N-terminal region; verify specificity in your system.
• Recommended for western blot detection of endogenous RGS5 (predicted MW ~21 kDa); consider using positive control lysates from tissues or cells expressing RGS5, such as vascular smooth muscle cells.
• For immunohistochemistry (paraffin-embedded sections), optimize antigen retrieval and antibody dilution; mouse cross-reactivity allows testing in murine models.
• This polyclonal antibody can be used in ELISA; ensure appropriate blocking and wash steps to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers and researchers with comprehensive access to IVD raw materials. For GPCR signaling studies, we offer a range of antibodies including this anti-RGS5 polyclonal antibody, along with matched pairs, recombinant proteins, and ancillary reagents. From concept to clinic, trust CamelBio to streamline your raw material sourcing needs.