Anti-RGS4 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P49798

Core Product Specifications and Parameters

Biological Background: RGS4 Function and Localization

• Regulator of G protein signaling 4 (RGS4) is a member of the RGS protein family that acts as a negative regulator of G protein-coupled receptor (GPCR) signaling.
• It functions as a GTPase-activating protein (GAP) for heterotrimeric G protein alpha subunits, enhancing GTP hydrolysis to return them to an inactive GDP-bound state.
• RGS4 preferentially modulates G(z)-alpha and G(i)-alpha subtypes; its activity can be inhibited by phosphorylation or palmitoylation of the target G protein alpha subunit.
• The RGS4 gene undergoes alternative splicing, generating multiple isoforms with distinct tissue distributions. Isoforms 4 and 5 are ubiquitously expressed, while isoforms 1, 2, and 3 are absent from the cerebellum.
• RGS4 is predominantly expressed in the brain (prefrontal and visual cortex) and heart at the protein level, aligning with its role in neural and cardiac signaling.
• The protein is classified as a phosphoprotein, lipoprotein, and palmitoylated protein, and has been associated with schizophrenia in genetic studies.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide covering amino acids 1–100 of human RGS4; this N-terminal region is relatively conserved across human, mouse, and rat, supporting cross-reactivity.
• For Western blot, the predicted molecular weight is ~23 kDa; consider loading brain or heart tissue lysates for robust signal detection.
• In immunofluorescence/ICC, optimize permeabilization and blocking conditions, and include a peptide blocking control or knockout cell line to confirm specificity.
• For ELISA, the antibody may be used as a capture or detection reagent; pairing with a matched monoclonal antibody can improve assay performance.
• Given the endogenous RGS4 expression in many neural tissues, validate the antibody in your specific tissue or cell type to rule out potential cross-reactivity with other RGS family members.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, supporting diagnostic manufacturers and researchers from early development to commercialization. Our RGS4 polyclonal antibody is part of a broader portfolio of signaling pathway reagents, and we offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. Contact us to streamline your procurement and accelerate your assay development.