Anti-RGS20 Rabbit Polyclonal Antibody for WB, ELISA - O76081

Core Product Specifications and Parameters

Biological Background: RGS20 Function and Localization

• RGS20 (Regulator of G protein signaling 20) is a member of the RGS protein family that functions as a GTPase-activating protein (GAP) for heterotrimeric G protein alpha subunits.
• It selectively binds to G(z)-alpha and G(alpha)-i2 subunits, accelerating their intrinsic GTPase activity and thereby driving G proteins into the inactive GDP-bound state, inhibiting signal transduction.
• RGS20 negatively regulates mu-opioid receptor-mediated activation of G proteins (by similarity).
• Its activity is regulated by phosphorylation and palmitoylation of the target G protein.
• The protein exists in multiple isoforms generated by alternative splicing; isoform 5 is specifically expressed in the brain, notably at high levels in the caudate nucleus and temporal lobe.
• Subcellularly, RGS20 localizes to the membrane, nucleus, and cytoplasm.
• Post-translational modifications include glycosylation, lipidation (palmitoylation), phosphorylation, and ubiquitin-like conjugation (Ubl conjugation), reflecting its dynamic regulation.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is ~44kDa; consider using appropriate tissue or cell lysates expressing RGS20, such as brain tissues, especially caudate nucleus or temporal lobe regions, based on isoform-specific expression.
• For ELISA, the recombinant immunogen can serve as a positive control to validate antibody binding.
• Since the antibody cross-reacts with mouse and rat, it is suitable for studies in rodent models; however, verify specificity in the relevant sample system.
• Given the polyclonal nature and immunogen design (full N-terminal fragment), the antibody may recognize multiple isoforms; reviewing the target sequence alignment across species and isoforms is advisable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, supporting every stage from concept to clinic. Our extensive portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for targets like RGS20, a key regulator in G protein signaling. Let our expert team streamline your procurement and accelerate your assay development.