Anti-RGR Polyclonal Antibody for WB, ELISA - P47804

Core Product Specifications and Parameters

Biological Background: RGR (RPE-retinal G protein-coupled receptor) Function and Localization

• RGR is a non-visual opsin that functions as a light-dependent facilitator of retinoid isomerization and trafficking in retinal pigment epithelium (RPE) and Mueller glia (PubMed:18474598).
  Related references: PMID:18474598
• Binds all-trans-retinal via a conserved Schiff-base lysine and, upon illumination, promotes photoisomerization to 11-cis-retinal required for visual pigment regeneration (By similarity).
• Mediates light-dependent mobilization of stored all-trans-retinyl esters from lipid droplets to endoplasmic reticulum membranes, facilitating their processing into 11-cis-retinoids (PubMed:18474598).
  Related references: PMID:18474598
• Subcellular localization: integral membrane protein.
• Preferentially expressed at high levels in the RPE and Mueller cells of the neural retina.
• Structurally a member of the G protein-coupled receptor (GPCR) family opsin, but lacks consistent evidence of G protein activation or canonical second-messenger signaling.
• Alternative splicing generates multiple isoforms; gene mutations are associated with retinitis pigmentosa.
• Keywords include photoreceptor protein, retinal protein, sensory transduction, transmembrane helix, and vision.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 156-295 of human RGR; epitopes are likely within this region. For blocking or competition studies, consider using the immunogen fragment.
• Antibody is validated for Western blot (WB) and ELISA. Optimize working dilutions for your specific sample type and experimental conditions.
• Cross-reactivity with rat suggests potential utility in rodent models; validate performance in your target tissue.
• RGR is a membrane protein; ensure thorough denaturation and use appropriate lysis buffers for WB analysis.
• As RGR functions in a light-dependent manner, consider controlling light exposure during sample preparation if studying its activation state.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering a comprehensive portfolio from validated antibodies to bulk ancillary reagents. Our platform connects diagnostic developers with critical tools for retinal and vision research, including targets like RGR. Explore our supply capabilities for antibody pairs, optimized monoclonals/polyclonals, and rare target sourcing to streamline your assay development pipeline.