Anti-RGL2 Rabbit Polyclonal Antibody for WB and ELISA - Q8GXW1

Core Product Specifications and Parameters

Biological Background: RGL2 (DELLA Protein) Function and Localization

• DELLA protein RGL2 (RGA-like 2) is a member of the GRAS family of plant-specific transcriptional regulators, also designated AtGRAS-15 or SCL19.
• Acts as a key repressor of gibberellin (GA) signaling by forming multiprotein complexes that inhibit transcription of GA-responsive genes; degraded via the proteasome upon GA perception, thereby triggering GA responses.
• Major repressor of seed germination, including thermoinhibition; promotes abscisic acid (ABA) biosynthesis in seed coats to maintain dormancy and mediates glucose-induced delay of germination (PubMed:24989044). Related references: PMID:24989044
• Delays flowering and vegetative phase change; regulates floral organ development (petals, stamens, anthers) by restraining growth; activity modulated by auxin and ethylene.
• Enhances salt tolerance by reducing stress-induced reactive oxygen species (ROS) accumulation and delaying ROS-mediated cell death; contributes to susceptibility to biotrophic pathogens and resistance to necrotrophs.
• Nucleus-localized transcriptional regulator.
• Expressed predominantly in germinating seeds, flowers, and siliques; found in seed coats, radicle, endosperm, and embryonic axis, but absent from leaves, stems, and roots.
• Post-translationally modified by phosphorylation and ubiquitin conjugation, impacting protein stability and activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant fragment spanning amino acids 40-156 of Arabidopsis RGL2 (NP_186995.1), which includes the DELLA domain; consider this sequence specificity when assessing cross-reactivity with other DELLA family members.
• For Western blot, a band around 60 kDa is expected; validate the detection in relevant tissue lysates (e.g., seed, flower bud) and consider using a dilution series to optimize signal-to-noise.
• ELISA protocols may benefit from coating with the recombinant immunogen or whole protein extracts; include appropriate negative controls (e.g., leaf tissue with low/absent expression).
• Because RGL2 is GA-regulated, carefully control plant growth conditions and hormone treatments; proteasome inhibitors can be used to assess GA-dependent degradation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic developers and researchers seamless access to a comprehensive range of IVD raw materials, including this Arabidopsis RGL2 antibody for studying gibberellin signaling and seed biology. Our portfolio encompasses validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets, supporting your project from discovery to clinical application. Partner with CamelBio to streamline your procurement and accelerate your plant science and agricultural biotechnology research.