Anti-RGAP1 Rabbit Monoclonal Antibody (KD Validated) for WB, IHC-P, ELISA - Q9H0H5

Core Product Specifications and Parameters

Biological Background: RGAP1 Function and Localization

• RGAP1, also known as Rac GTPase-activating protein 1, MgcRacGAP, or CYK4, is a 71 kDa component of the centralspindlin complex, a microtubule-dependent and Rho-mediated signaling module that orchestrates contractile ring formation during cytokinesis. It sequentially binds ECT2 and RAB11FIP3 to regulate cleavage furrow ingression and abscission. Related references: PMID:18511905
• Beyond its GAP activity, RGAP1 controls growth and differentiation of hematopoietic cells through mechanisms independent of Rac GTPase regulation. Related references: PMID:10979956
• RGAP1 plays a critical role in erythropoiesis, and mutations in the RACGAP1 gene are associated with congenital dyserythropoietic anemia type IIIB (CDAN3B). Related references: PMID:34818416
• RGAP1 dynamically localizes to the nucleus, cytoplasm, spindle, cleavage furrow, midbody, midbody ring, and cell membrane, reflecting its multifaceted roles during cell division.
• Highly expressed in testis, thymus, and placenta, with lower levels in spleen and peripheral blood lymphocytes. In testis, expression is restricted to germ cells, peaking in spermatocytes. Expression is cell cycle-dependent, peaking in G2/M phase.
• RGAP1 exhibits strong GAP activity toward CDC42 and RAC1, and weaker activity toward RHOA. It may also participate in sulfate transport in male germ cells, spermatogenesis, and neuronal proliferation.
• The protein contains a zinc-finger and coiled-coil domain, and undergoes acetylation, phosphorylation, and ubiquitination, indicating complex regulation.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide spanning amino acids 533–632; this region may be accessible in native and denatured conformations, supporting applications such as WB and IHC-P. Validate in the relevant sample system.
• For Western blot, consider lysates from cell lines with moderate to high RGAP1 expression (e.g., HeLa, Jurkat) or testis tissue. RGAP1 expression peaks in G2/M phase; synchronization may enhance signal.
• For IHC-P, verify staining in formalin-fixed, paraffin-embedded sections of testis, thymus, or placenta, where RGAP1 is abundantly expressed. Antigen retrieval conditions may require optimization.
• The antibody cross-reacts with mouse and rat RGAP1; validate species-specific reactivity using appropriate tissue or cell controls.
• ELISA can be performed using the immunogen peptide as a positive control; antibody titer and binding affinity should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material procurement partner, streamlining access to critical reagents for diagnostic developers. This monoclonal antibody targets RGAP1, a pivotal player in cell division and erythropoiesis with relevance to hematological disorder research and assay development. Through its one-stop platform, CamelBio can support your projects with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare target raw materials—empowering your transition from concept to clinic.