Anti-RGA1 (GPA1) Polyclonal Antibody for WB, ELISA - Q0DJ33

Core Product Specifications and Parameters

Biological Background: RGA1 (GPA1) Function and Localization

• Guanine nucleotide-binding protein alpha-1 subunit (GPA1), also known as RGA1, Dwarf1, or Daikoku dwarf protein, is the α subunit of heterotrimeric G proteins in rice (Oryza sativa).
• It functions as a modulator or transducer in various transmembrane signaling systems, essential for normal growth and development of internodes, leaves, panicles, and seeds. Related references: PMID:10377457
• Involved in gibberellin signal transduction, contributing to plant hormone responses. Related references: PMID:10468600 PMID:11027362
• Plays a role in R gene-mediated disease resistance, functioning upstream of the small GTPase RAC1 in early signaling steps. Related references: PMID:12237405
• Participates in brassinosteroid (BR) response, possibly acting together with the E3 ubiquitin ligase TUD1 to mediate a BR signaling pathway affecting plant growth and development. Related references: PMID:17117160 PMID:19036785 PMID:23526892
• Localized to the cell membrane via lipid anchors (myristate, palmitate).
• Tissue-specifically expressed: highly in young internodes, stamen, pistil, lemma, palea, and pericarp of ovary after fertilization.
• Belongs to the G-alpha family; possesses GTP-binding, hydrolase activity, and requires magnesium as a cofactor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 22-230 of Oryza sativa RGA1 (Q0DJ33). This region may include functionally important domains; users should validate antibody specificity in relevant rice tissue lysates.
• For Western blot (WB), consider using young internode or panicle extracts where RGA1 is highly expressed according to tissue specificity data.
• ELISA application may be suitable for detecting recombinant or native RGA1; appropriate controls should be included to assess signal-to-noise ratios.
• Cross-reactivity is reported only for Oryza sativa; reactivity with orthologs in other plant species is not characterized and should be tested individually.
• The predicted molecular weight is 44 kDa, but post-translational modifications (e.g., lipidation) may alter migration on SDS-PAGE; use appropriate molecular weight markers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined sourcing solution for IVD raw materials, including validated antibodies and ancillary reagents. This anti-RGA1 polyclonal antibody supports research into rice developmental signaling and hormone pathways. Beyond individual antibodies, CamelBio can supply optimized antibody pairs, bulk recombinant proteins, and rare-target sourcing for plant biology studies, helping diagnostic manufacturers and researchers accelerate their workflows from concept to clinical application.