Anti-RFX3 Rabbit Polyclonal Antibody for WB, ELISA - P48380

Core Product Specifications and Parameters

Biological Background: RFX3 Function and Localization

• RFX3 is a transcription factor belonging to the regulatory factor X family, with alternative splicing producing multiple isoforms.
• Essential for ciliogenesis: required for differentiation of nodal monocilia and left-right asymmetry specification, and for biogenesis of motile cilia by governing growth and beating efficiency.
• Regulates ciliary gene expression, including DYNC2LI1, FOXJ1, and BBS4 for assembly, and DNAH11, DNAH9, and DNAH5 for motility.
• Participates in endocrine pancreas development together with RFX6, driving differentiation of most islet cell types except pancreatic polypeptide-producing cells.
• Forms heterodimers with other RFX proteins and binds X-box promoter elements to regulate transcription. Related references: PMID:20148032
• Represses transcription of MAP1A specifically in non-neuronal cells, indicating cell-type-specific regulation. Related references: PMID:12411430
• Subcellular localization is strictly nuclear, consistent with its role as a transcription factor.
• Required for differentiation of ciliated ependymal cells.

Experimental Guidance and Technical Tips

• The immunogen was a recombinant fusion protein covering amino acids 280–430 of human RFX3; this region may be shared across isoforms, but validation in your specific experimental system is advised.
• For Western blotting (WB), the expected band is around 84 kDa; use appropriate positive controls (e.g., RFX3-expressing cell lines) and confirm with siRNA knockdown or overexpression.
• In ELISA applications, titrate the antibody with recombinant RFX3 protein to establish sensitivity and linear range.
• As a polyclonal antibody, batch-to-batch variability may occur; consistent lot performance should be confirmed for critical assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-RFX3 rabbit polyclonal antibody is ideally suited for research into ciliogenesis, transcriptional regulation, and pancreatic development. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to accelerate your next diagnostic breakthrough.