Anti-RFX2 Polyclonal Antibody for WB, ELISA - P48378

Core Product Specifications and Parameters

Biological Background: RFX2 Function and Localization

• RFX2, also known as Regulatory factor X 2, is a DNA-binding transcription factor that specifically recognizes and binds the X-box regulatory motif (consensus: 5'-GTNRCC(0-3N)RGYAAC-3') (PubMed:10330134). Related references: PMID:10330134
• It acts as a key regulator of spermatogenesis, controlling the expression of genes required for the haploid phase during spermiogenesis, including those critical for cilium assembly and function.
• RFX2 likely activates transcription of the testis-specific histone H1-6 gene, contributing to chromatin remodeling during spermatogenesis (By similarity).
• Alternative splicing generates multiple isoforms of RFX2, potentially expanding its functional repertoire in gene regulation.
• Subcellular localization studies reveal RFX2 in both the nucleus and cytoplasm, indicating dynamic intracellular trafficking or compartment-specific activities.
• Post-translational modifications, such as phosphorylation identified by proteomics, may modulate RFX2 stability, DNA-binding affinity, or transcriptional activity.
• Associated keywords include DNA-binding, transcription, transcription regulation, spermatogenesis, cilium biogenesis/degradation, differentiation, and phosphoprotein, underscoring its multifaceted biological roles.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 295-450) targets a central region of human RFX2; given its cross-reactivity with mouse, consider verifying specificity in the intended species using appropriate controls.
• For Western blotting, the expected molecular weight is approximately 80 kDa; optimize sample preparation and loading conditions to detect endogenous RFX2 levels.
• In ELISA applications, the antibody can be used as a capture or detection reagent; validate optimal coating concentrations and detection methods for your specific assay format.
• As a transcription factor, RFX2 abundance may be low in some cell types; consider enriching for nuclear extracts if whole cell lysates yield weak signals.
• Due to alternative splicing, multiple bands may appear in WB; confirm band identity using overexpression or knockdown controls if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as an integrated sourcing partner, delivering comprehensive IVD raw-material solutions from discovery to commercialization. Supporting research in reproductive biology and transcriptional regulation, we supply validated antibody pairs, optimized polyclonal antibodies like this anti-RFX2 antibody, and bulk ancillary reagents. Our team also excels in sourcing rare target raw materials, accelerating your diagnostic development pipeline.