Anti-RFWD2/COP1 Rabbit Polyclonal Antibody for WB, ELISA - Q8NHY2

Core Product Specifications and Parameters

Biological Background: RFWD2 (COP1) Function and Localization

• E3 ubiquitin-protein ligase COP1 (also known as RFWD2, RNF200, hCOP1) mediates ubiquitination and subsequent proteasomal degradation of key regulatory proteins, including p53 (TP53), JUN, SFN (14-3-3 sigma), and MTA1.
• By targeting p53 for degradation independently of MDM2 and RCHY1, COP1 abolishes p53-dependent transcription and apoptosis, promoting cell survival.
• The protein contains a RING finger domain (zinc-binding) and WD repeats, and can function as the essential RING subunit in certain E3 ligase complexes; however, in the DCX DET1-COP1 complex, the catalytic RING activity is provided by RBX1.
• Ubiquitination of SFN leads to AKT activation and enhanced cell survival, while degradation of MTA1 further underscores its role in transcriptional regulation.
• In response to TRIB1 binding, COP1 can also ubiquitinate CEBPA via a non-canonical binding motif.
• COP1 localizes predominantly to nuclear speckles and the cytoplasm, consistent with its roles in transcription factor regulation.
• It is ubiquitously expressed at low levels, with higher expression in testis, placenta, skeletal muscle, and heart.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 582-731 of human RFWD2, a region likely to recognize full-length COP1 (predicted MW ~80 kDa). Consider using a whole-cell lysate from human cell lines for Western blot verification.
• For ELISA, the polyclonal nature may provide robust detection; titrate the antibody and validate with recombinant COP1 protein or known positive samples.
• Cross-reactivity is confirmed for human; reactivity with other species is not specified—validate in the relevant sample system if needed.
• Optimal working dilutions and conditions should be determined empirically by the end user.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For targets like RFWD2/COP1 involved in ubiquitination and cancer pathways, we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist with rare target raw-material sourcing. Contact us to explore how our integrated supply chain can accelerate your assay development and manufacturing workflows.