Anti-RFPL2 Rabbit Polyclonal Antibody for WB, ELISA - O75678

Core Product Specifications and Parameters

Biological Background: RFPL2 (Ret finger protein-like 2) Function and Localization

• RFPL2 (Ret finger protein-like 2), also designated as RING finger protein 79 (RNF79), is a zinc-finger protein predicted to contain a RING domain, suggesting a role in ubiquitin ligation and protein turnover.
• The RFPL2 gene produces multiple transcript variants through alternative splicing, potentially yielding isoforms with distinct regulatory properties.
• Based on tissue-specific expression data, RFPL2 is primarily detected in the prostate, with lower expression in adult brain, fetal liver, and fetal kidney, indicating a role in urogenital and neural development.
• The protein is classified under the metal-binding and zinc-finger categories, implying coordination of zinc ions for structural integrity and function.
• No post-translational modifications are currently annotated for RFPL2 in UniProt.

Experimental Guidance and Technical Tips

• For Western blotting, a band near 42 kDa is expected for the canonical isoform. Prestained markers around this range are recommended.
• In ELISA, the antibody may be used as a capture or detection reagent depending on the assay format; optimization of coating concentration and detection system is advised.
• When working with rat samples, validate cross-reactivity by comparing human and rat tissue lysates, as sequence homology in the immunogen region may influence detection sensitivity.
• The immunogen peptide spans a region within amino acids 50-150; confirming target specificity via peptide blocking or siRNA knockdown in the relevant cell line is suggested.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For zinc-finger protein research and prostate biomarker development, CamelBio can source validated RFPL2 antibody pairs, optimized polyclonal/monoclonal antibodies, and ancillary reagents in bulk, alongside customized solutions for rare target raw-material procurement.