Anti-RFK Rabbit Polyclonal Antibody for WB and ELISA - Q969G6

Core Product Specifications and Parameters

Biological Background: RFK Function and Localization

• Riboflavin kinase (RFK), also known as flavokinase or ATP:riboflavin 5'-phosphotransferase, is the enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to produce flavin mononucleotide (FMN), a key step in the biosynthesis of FAD.
• RFK is the rate-limiting enzyme in FAD synthesis, essential for cellular metabolism and flavoprotein function.
• It plays a critical role in TNF-induced reactive oxygen species (ROS) production by physically and functionally coupling the TNF receptor (TNFRSF1A) to NADPH oxidase via interaction with CYBA.
• RFK is a cytoplasmic protein, consistent with its role in metabolic pathways.
• The protein contains ATP-binding, nucleotide-binding, and metal-binding motifs (Mg²⁺ and Zn²⁺), classifying it as a kinase/transferase.
• Tissue expression has been detected in brain, placenta, and urinary bladder.
• Additional structural and functional annotations include 3D-structure, flavoprotein, FMN, and reference proteome keywords.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant full-length human RFK protein (aa 1-155), which should generate antibodies recognizing multiple linear and conformational epitopes.
• For Western blotting, a specific band around 18 kDa is anticipated; we recommend titrating the primary antibody (e.g., 1:500–1:2000) and using appropriate positive controls (brain or placenta lysates) and negative controls.
• When performing ELISA, the antibody can be used as a capture or detection reagent; optimization of coating concentration and secondary antibody is advised.
• Cross-reactivity with mouse RFK is expected based on sequence homology; validate specificity for your sample species.
• Use standard rabbit secondary antibodies and detection systems; store aliquots at -20°C to avoid freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive raw-material solutions for IVD manufacturers and researchers, including validated antibodies, antigens, and ancillary reagents. For riboflavin kinase and related metabolic targets, we offer ready-to-use polyclonal and monoclonal antibodies, supporting your assay development from initial research to clinical application. Contact us to source custom antibody pairs or bulk quantities to accelerate your diagnostic pipeline.