Anti-RFC5 Polyclonal Antibody for WB, ELISA - P40937

Core Product Specifications and Parameters

Biological Background: Replication factor C subunit 5 Function and Localization

• RFC5 encodes the 36 kDa subunit of the replication factor C (RFC) complex, also designated activator 1 subunit 5 or RFC36.
• As part of the RFC clamp loader, it mediates the ATP-dependent loading of proliferating cell nuclear antigen (PCNA) onto primed DNA, a critical step for DNA replication.
• PCNA loading enables processive DNA synthesis by DNA polymerases delta and epsilon during the elongation phase.
• RFC5 is primarily localized to the nucleus, consistent with its function in DNA metabolism.
• Post-translational modifications include acetylation, and the gene undergoes alternative splicing.
• The protein is classified as a reference proteome component with proteomics identification support.
• Its molecular weight is approximately 38.5 kDa, aligning with the antibody's target range.

Experimental Guidance and Technical Tips

• The immunogen, corresponding to amino acids 181–340 of human RFC5, is predicted to be conserved across species; test cross-reactivity in mouse and rat samples as indicated.
• For Western blotting, consider testing a dilution range of 1:500–1:2000 using nuclear extracts or whole-cell lysates from proliferating cell lines.
• Validate the antibody in ELISA by coating with recombinant RFC5 protein or the immunogen peptide under appropriate buffer conditions.
• Include appropriate controls (e.g., RFC5 knockdown or knockout cell lysates) to confirm band specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD raw materials, providing validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our RFC5 antibody supports research in DNA replication, cell cycle regulation, and cancer biology. Contact us for customized sourcing solutions, including rare target raw materials and technical consultation.