Anti-RFC3 Polyclonal Antibody for WB and ELISA - P40938

Core Product Specifications and Parameters

Biological Background: RFC3 Function and Localization

• RFC3 is the 38 kDa subunit of the replication factor C (RFC) complex, also known as Activator 1 subunit 3.
• It is essential for ATP-dependent loading of proliferating cell nuclear antigen (PCNA) onto primed DNA, facilitating DNA replication by polymerases delta and epsilon.
• The RFC complex acts during the elongation phase of DNA replication on primed templates.
• Localizes primarily to the nucleus.
• Contains acetylation and phosphorylation sites that may regulate its activity.
• Undergoes alternative splicing to produce multiple isoforms.
• The protein has been characterized by 3D-structure determination, aiding functional studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-280, covering the N-terminal region of human RFC3; consider this when designing detection strategies.
• For Western blot, the expected molecular weight is approximately 41 kDa; validate in relevant sample systems such as nuclear extracts from human, mouse, or rat cells.
• Due to alternative splicing, multiple bands may be observed; confirm specificity using appropriate controls.
• In ELISA applications, include recombinant protein or cell lysate standards and perform thorough validation.
• As a polyclonal antibody, lot-to-lot consistency should be monitored; consider including internal reference samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This RFC3 antibody supports investigations into DNA replication and cell cycle research. From rare target sourcing to custom development, CamelBio bridges your project from concept to clinic with reliable, scalable supply solutions.