Anti-RFC1 Rabbit Polyclonal Antibody for WB, IF/ICC and ELISA - P35251

Core Product Specifications and Parameters

Biological Background: RFC1 Function and Localization

• RFC1 encodes the large subunit (140 kDa) of the heteropentameric replication factor C (RFC) complex, also known as activator 1.
• The RFC complex is required for elongation of primed DNA templates by DNA polymerases delta and epsilon, and loads PCNA onto DNA in an ATP-dependent manner (PubMed:9488738).
  Related references:
  PMID:9488738
• This subunit specifically binds the primer-template junction and can interact with both single- and double-stranded DNA, as well as GA-rich sequences including the PO-B transcription element, suggesting dual roles in DNA replication and transcription regulation.
• RFC1 is localized to the nucleus and is widely expressed across human tissues except placenta.
• The protein is subject to post-translational modifications, including phosphorylation and ubiquitination (isopeptide bond), as indicated by large-scale proteomic studies.
• Mutations in RFC1 are associated with cerebellar ataxia, neuropathy, vestibular areflexia syndrome (CANVAS), expanding its clinical relevance beyond core DNA metabolism.
• Alternative splicing generates multiple transcript variants, contributing to functional diversity.

Experimental Guidance and Technical Tips

• For Western blot, expect a band around 128 kDa. Use 6-8% SDS-PAGE for optimal separation of high-molecular-weight proteins.
• For immunofluorescence/ICC, include a nuclear counterstain (e.g., DAPI) and confirm nuclear localization. Permeabilization conditions may need optimization.
• ELISA: The antibody may be used to detect recombinant or endogenous RFC1; titrate appropriately.
• Sequence alignment of the immunogen (aa 400-700) across human, mouse, and rat indicates high conservation, supporting reported cross-reactivity; however, validate in your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with a one-stop channel for high-quality IVD raw materials. Our portfolio includes this anti-RFC1 polyclonal antibody, validated for applications such as WB and IF, alongside curated reagent panels for DNA replication and repair studies. We also offer bespoke sourcing for rare targets, bulk reagents, and technical consulting to help streamline your assay development from concept to clinic.