Anti-Rev-Erbα/NR1D1 Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, ELISA - P20393

Core Product Specifications and Parameters

Biological Background: Rev-Erbα/NR1D1 Function and Localization

• Rev-Erbα (NR1D1) is a heme-binding nuclear receptor that acts as a transcriptional repressor, forming a critical negative limb of the circadian clock by directly repressing core clock genes including BMAL1, CLOCK, and CRY1.
• It regulates diverse metabolic pathways, including lipid and bile acid metabolism, adipogenesis, gluconeogenesis, and inflammatory responses, in a heme-dependent manner by recruiting the NCOR1/HDAC3 corepressor complex.
• The protein binds to DNA as a monomer at RevRE elements (consensus 5'-[A/G]GGTCA-3' with an A/T-rich 5' sequence) or as a homodimer at RevDR-2 elements (direct repeats spaced by two nucleotides).
• Rev-Erbα competes with RORα for DNA binding and represses PPARGC1A, thereby controlling heme synthesis and rhythmic expression of metabolic enzymes.
• Subcellularly, NR1D1 is primarily nuclear but also localizes to the cytoplasm and is found in dendritic spines, consistent with its role in neuronal functions.
• Widely expressed with high levels in liver, adipose tissue, skeletal muscle, brain, and macrophages; expression oscillates diurnally in the suprachiasmatic nucleus and peripheral tissues.
• Key post-translational modifications include phosphorylation, acetylation, and ubiquitination, which modulate its stability and activity; ubiquitin-mediated degradation relieves inflammatory repression upon triggers.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 280–360 within the ligand-binding domain; this region may confer specificity for the receptor's conformation and heme-dependent interactions.
• For Western blotting, the predicted molecular weight is 67 kDa; verify band size in relevant samples, as endogenous expression may vary with circadian time and tissue type.
• In immunofluorescence/immunocytochemistry (IF/ICC), anticipate nuclear predominant staining; however, cytoplasmic and dendritic localization may be observed in neuronal cells, so appropriate fixation and permeabilization conditions should be optimized.
• For ELISA applications, consider pairing with a detection system that recognizes the rabbit IgG Fc region if using this primary antibody in an indirect format.
• When working with Rev-Erbα, note that its expression and activity are rhythmically regulated; harvest samples at consistent circadian time points to ensure reproducibility.
• The antibody cross-reacts with mouse and rat, so it can be used for comparative studies across species; validate in the desired species by appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated partner for IVD raw-material procurement, offering a streamlined supply of validated antibodies and reagents for circadian rhythm and metabolic disease research. Our portfolio includes optimized monoclonal antibodies like this Rev-Erbα/NR1D1 rabbit mAb, along with antibody pairs, bulk ancillary materials, and sourcing support for rare targets, empowering diagnostic developers and researchers from concept to clinic.