Anti-Rev-erb beta/NR1D2 Polyclonal Antibody for WB, ELISA - Q14995

Core Product Specifications and Parameters

Biological Background: Rev-erb beta/NR1D2 Function and Localization

• NR1D2 (Rev-erb beta) is a heme-binding nuclear receptor that functions primarily as a transcriptional repressor, playing a central role in the circadian clock and metabolic regulation.
• It forms a critical negative limb of the circadian oscillator by directly repressing the expression of core clock components BMAL1 and CLOCK.
• In metabolic tissues, it regulates lipid and energy homeostasis by repressing genes such as CD36, FABP3, FABP4, UCP3, SCD1, and APOC3, and can also activate SREBF1 and IL6 in skeletal muscle.
• DNA binding occurs either as a monomer to a RevRE (5'-PuGGTCA-3' preceded by an A/T-rich sequence) or as a homodimer to a direct repeat spaced by two nucleotides (RevDR-2), allowing competitive repression of RORA.
• Beyond circadian and metabolic functions, it contributes to the regulation of the sleep/wake cycle and may influence neutrophilic inflammation in the lung.
• The protein is primarily nuclear but can also localize to the cytoplasm; it is widely expressed with highest levels in liver, adipose tissue, skeletal muscle, and brain, and its expression oscillates diurnally in the suprachiasmatic nucleus.
• Key structural features include zinc-finger motifs for DNA binding, and post-translational modifications such as acetylation, phosphorylation, and sumoylation regulate its activity.

Experimental Guidance and Technical Tips

• The immunogen targets a region within the hinge/ligand-binding domain (aa 220–300) of human NR1D2, which may generate antibodies that detect both monomeric and DNA-bound forms of the protein. For WB, consider using nuclear extracts or whole-cell lysates from tissues with high expression, such as liver or skeletal muscle.
• Given the listed applications (WB and ELISA), optimize antibody dilutions and validate performance in your specific experimental system. Cross-reactivity with mouse and rat makes this antibody suitable for studies in rodent models of circadian or metabolic disorders.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, laboratories, and research institutes to provide comprehensive IVD raw material sourcing. This anti-Rev-erb beta/NR1D2 antibody supports research in circadian biology, metabolic disorders, and inflammation. We offer one-stop access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, helping you streamline your workflow from discovery to clinical application.