Anti-Rev-erb beta/NR1D2 Monoclonal Antibody for WB, ELISA - Q14995

Core Product Specifications and Parameters

Biological Background: Rev-erb beta/NR1D2 Function and Localization

• Rev-erb beta, encoded by the NR1D2 gene, is a member of the nuclear receptor subfamily 1 group D. It functions as a heme-dependent transcriptional repressor and activator that integrates circadian and metabolic pathways.
• As a core component of the circadian clock, Rev-erb beta directly represses the expression of BMAL1 and CLOCK, forming a critical negative feedback loop that sustains 24-hour rhythmicity.
• The protein binds to two classes of DNA response elements (RevRE and RevDR-2) as a monomer or homodimer, allowing competition with ROR alpha (RORA) and regulation of genes involved in lipid metabolism and inflammation.
• Rev-erb beta acts as a receptor for heme, which stimulates interaction with the NCOR1/HDAC3 corepressor complex to enhance transcriptional repression of target genes.
• Subcellular localization is primarily nuclear, but it has also been detected in the cytoplasm, reflecting dynamic shuttling linked to its regulatory functions.
• Widely expressed with high levels in liver, adipose tissue, skeletal muscle, and brain, its expression oscillates diurnally in the suprachiasmatic nucleus (SCN) and peripheral tissues.
• Key post-translational modifications include acetylation and phosphorylation, which modulate its activity, and it contains zinc-finger motifs critical for DNA binding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 220-300 in the ligand-binding domain, suggesting the antibody recognizes native Rev-erb beta; confirm specificity by using positive and negative lysates in WB.
• For WB, expect a band near 65 kDa. Due to potential post-translational modifications, a minor shift in apparent molecular weight may occur; validate in your specific sample system.
• Cross-reactivity with mouse and rat makes this antibody suitable for comparative studies; verify reactivity in your models, as expression levels may differ.
• For ELISA, optimize coating conditions and antibody dilutions; the recombinant immunogen can serve as a positive control to establish assay sensitivity.
• Given Rev-erb beta's circadian oscillation, consider harvesting samples at multiple time points to capture temporal changes in target abundance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw-material sourcing, supporting research on nuclear receptors like Rev-erb beta with a comprehensive portfolio of validated antibodies, recombinant proteins, and ancillary reagents. Whether you need bulk monoclonal antibodies, matched pairs for diagnostic kit development, or assistance in sourcing rare-target materials, our supply network streamlines production from concept to clinic. We help diagnostic manufacturers and laboratories secure reliable, high-quality raw materials to accelerate circadian rhythm and metabolic disease research.