Anti-RERE (Atrophin-1-like Protein) Polyclonal Antibody for WB and ELISA - Q9P2R6

Core Product Specifications and Parameters

Biological Background: RERE Function and Localization

• RERE, also known as Atrophin-1-like protein (ARG/ARP), is an arginine-glutamic acid dipeptide repeat-containing transcriptional repressor.
• It plays critical roles in developmental processes and may influence cell survival pathways.
• Overexpression of RERE can recruit BAX to nuclear PODs and trigger caspase-3 activation, leading to apoptosis.
• The protein localizes primarily in the nucleus, consistent with its function in transcriptional regulation.
• RERE is widely expressed in normal tissues and is also detected in various tumor cell lines.
• It undergoes multiple post-translational modifications, including acetylation, phosphorylation, and ubiquitination, and contains zinc-finger domains for metal binding.
• The RERE gene is subject to alternative splicing, potentially producing multiple isoforms.
• It belongs to the atrophin family of developmental regulators and transcriptional repressors.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is approximately 172 kDa; however, actual migration may differ due to post-translational modifications. Validate using appropriate positive and negative controls in human, mouse, or rat samples.
• The polyclonal antibody was raised against an N-terminal recombinant fragment (aa 1-220), which may favor recognition of denatured epitopes. When performing native ELISA, confirm reactivity under your specific assay conditions.
• As with any polyclonal antibody, lot-to-lot variability may occur. Include internal reference standards and consider pre-absorption controls if non-specific binding is observed.
• This antibody may be suitable for indirect ELISA as a primary antibody. For sandwich ELISA, pairing with a well-characterized matched antibody is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, providing diagnostic manufacturers, laboratories, and research institutes with access to validated antibodies, bulk ancillary reagents, and rare target raw-material sourcing. For RERE-related research in transcriptional regulation and apoptosis, our portfolio includes optimized polyclonal and monoclonal antibodies, as well as flexible supply options to support your assay development from concept to clinic.