Anti-Reptin/RUVBL2 Rabbit Monoclonal Antibody for WB, ELISA - Q9Y230

Core Product Specifications and Parameters

Biological Background: Reptin/RUVBL2 (RuvB-like 2) Function and Localization

• Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity; hexamerization is critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity. Related references: PMID:10428817, PMID:17157868, PMID:33205750
• Component of the NuA4 histone acetyltransferase complex, which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A; this complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. Related references: PMID:14966270
• Component of a SWR1-like complex that specifically mediates the removal of histone variant H2A.Z/H2AZ1 from the nucleosome. Related references: PMID:24463511
• Proposed core component of the chromatin remodeling INO80 complex which exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding. Related references: PMID:16230350, PMID:21303910
• Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. Related references: PMID:10882073, PMID:16014379
• May also inhibit the transcriptional activity of ATF2. Related references: PMID:11713276
• Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway where it negatively regulates expression of ER stress response genes. Related references: PMID:25652260
• Localizes to nucleus matrix, nucleoplasm, cytoplasm, membrane, and dynein axonemal particles; ubiquitously expressed with highest levels in testis and thymus. Undergoes alternative splicing to produce multiple isoforms.

Experimental Guidance and Technical Tips

• For western blot (WB), consider optimizing antibody dilution and blocking conditions using a validated positive control (e.g., HeLa or HEK293 whole-cell lysate); the predicted protein weight is ~51 kDa.
• The mouse cross-reactivity makes this antibody suitable for studies in mouse models; validate in the relevant mouse tissue or cell line to confirm reactivity.
• For ELISA applications, titrate the antibody against the recombinant protein or the immunizing peptide; this can be used as a capture or detection reagent in indirect or sandwich ELISA setups.

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CamelBio offers a comprehensive range of IVD raw materials, technical services, and consulting to support your research and diagnostic development from concept to clinic. For chromatin remodeling, transcription regulation, and DNA repair studies, we provide validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. Our platform also helps source rare-target raw materials, ensuring you have uninterrupted access to critical reagents for your assays.