Anti-Reptin/RUVBL2 Polyclonal Antibody for WB, ELISA - Q9Y230

Core Product Specifications and Parameters

Biological Background: Reptin/RUVBL2 Function and Localization

• Reptin/RUVBL2 is a DNA-dependent ATPase and 5'-3' DNA helicase, with hexamerization essential for ATP hydrolysis and cooperative ATPase activity across adjacent subunits.
  Related references: PMID:10428817 | PMID:17157868 | PMID:33205750
• Component of the NuA4 histone acetyltransferase complex, which activates transcription by acetylating nucleosomal histones H4 and H2A, altering nucleosome-DNA interactions and facilitating DNA repair recruitment.
  Related references: PMID:14966270
• Proposed core component of the INO80 chromatin remodeling complex, which exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding.
  Related references: PMID:16230350 | PMID:21303910
• Functions in a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome.
  Related references: PMID:24463511
• Plays an essential role in oncogenic transformation by MYC and modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex; may also inhibit ATF2 transcriptional activity.
  Related references: PMID:10882073 | PMID:16014379 | PMID:11713276
• Involved in the endoplasmic reticulum-associated degradation (ERAD) pathway, negatively regulating expression of ER stress response genes.
  Related references: PMID:25652260
• May regulate the composition of the U5 snRNP complex, impacting pre-mRNA splicing.
  Related references: PMID:28561026
• Subcellular localization includes the nucleus matrix, nucleoplasm, cytoplasm, membrane, and dynein axonemal particles, with ubiquitous expression and enrichment in testis and thymus.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin conjugation (isopeptide bond), which regulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering the full-length Reptin/RUVBL2 (amino acids 1-463), suggesting the antibody recognizes total protein; validate endogenous expression in relevant cell lines or tissues.
• For Western blot, a band around 51 kDa is expected; consider using a loading control and optimizing antibody dilution and blocking conditions for your specific samples.
• This polyclonal antibody has been reported to cross-react with human, mouse, and rat; perform species-specific validation if using beyond these species.
• In ELISA applications, the antibody may serve as a capture or detection reagent; test serial dilutions and confirm linearity and sensitivity with your target antigen.
• As a polyclonal preparation, potential lot-to-lot variability exists; include appropriate positive and negative controls and re-validate when switching lots.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering a broad range of validated antibodies, recombinant proteins, and ancillary reagents to support diagnostic development from concept to clinic. The Reptin/RUVBL2 polyclonal antibody is part of our chromatin biology and oncology portfolio, enabling research into transcriptional regulation and DNA repair pathways. Contact us for bulk supplies, custom antibody pairs, or technical assistance in sourcing rare-target materials.