Anti-REPIN1 Rabbit Monoclonal Antibody for WB - Q9BWE0

Core Product Specifications and Parameters

Biological Background: REPIN1 Function and Localization

• DNA-binding protein REPIN1 acts as a sequence-specific double-stranded DNA-binding protein that preferentially recognizes ATT-rich and T-rich sequences and facilitates DNA bending (PubMed:10606657, PubMed:11328883, PubMed:2174103). Related references: PMID:10606657, PMID:11328883, PMID:2174103
• It may regulate the expression of genes involved in cellular fatty acid import, such as SCARB1/CD36, and genes linked to lipid droplet formation.
• REPIN1 may also influence iron metabolism and apoptosis-related pathways by regulating LCN2 expression.
• It might be involved in the control of glucose transport genes.
• The protein localizes to the nucleus, cytoplasm, and cytosol, suggesting dynamic shuttling or multiple functional compartments.
• It is expressed in adipose tissue and bone tissue, consistent with metabolic and structural roles.
• Post-translationally, REPIN1 is subject to acetylation and phosphorylation, potentially modulating its activity and interactions.
• Structurally, it contains zinc-finger motifs, indicating direct contact with DNA.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fragment corresponding to amino acids 376–567 of human REPIN1; epitope mapping may help confirm binding regions.
• For Western blot analysis, REPIN1 has a predicted molecular weight of 64 kDa; consider using a 10% SDS-PAGE gel and validate band size with appropriate controls.
• In immunohistochemistry (paraffin-embedded), REPIN1 expression is reported in adipose and bone tissues; these may serve as positive controls for initial validation.
• When using ELISA, include recombinant REPIN1 protein as a standard to assess linearity and sensitivity.
• As the antibody shows cross-reactivity with human REPIN1, confirm reactivity in the relevant species before proceeding with non-human models.
• Consider evaluating potential off-target binding by probing lysates with overexpressed and knockdown REPIN1 to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This REPIN1 monoclonal antibody supports metabolic and lipid disorder research, and CamelBio can further supply validated antibody pairs, optimized bulk reagents, and rare target sourcing to accelerate your assay development and scale-up.