Anti-REPIN1 Polyclonal Antibody for WB, ELISA - Q9BWE0

Core Product Specifications and Parameters

Biological Background: REPIN1 Function and Localization

• REPIN1 (Replication initiator 1), also known as RIP60, ZNF464, or 60 kDa origin-specific DNA-binding protein, is a zinc-finger protein with sequence-specific DNA-binding properties.
• It binds ATT-rich and T-rich DNA sequences and facilitates DNA bending (PubMed:10606657, PubMed:11328883, PubMed:2174103). Related references: PMID:10606657 PMID:11328883 PMID:2174103
• REPIN1 may regulate the expression of genes involved in cellular fatty acid import, including SCARB1/CD36, and genes involved in lipid droplet formation, suggesting a role in lipid metabolism.
• It may also regulate LCN2 expression, thereby influencing iron metabolism and apoptosis-related pathways.
• Additionally, REPIN1 may regulate genes involved in glucose transport, pointing to a broader role in metabolic homeostasis.
• The protein localizes to the nucleus, cytoplasm, and cytosol, indicating dynamic subcellular distribution.
• It is expressed in adipose tissue and bone tissue, as per tissue specificity data.
• Post-translational modifications include acetylation and phosphorylation, according to UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 376–567 of human REPIN1. This region may produce antibodies recognizing both human and mouse REPIN1, consistent with observed cross-reactivity.
• For Western blot, use standard protein extraction and blocking protocols. Optimize antibody concentration for your specific sample type and detection system.
• For ELISA, consider direct or indirect formats and perform checkerboard titrations to determine optimal coating antigen and antibody concentrations.
• As a polyclonal antibody, it may detect multiple epitopes, potentially yielding strong signals but requiring careful validation for specificity in your experimental context.
• Given REPIN1's presence in nucleus and cytoplasm, subcellular fractionation may be necessary for localization studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials, including this REPIN1 polyclonal antibody for studies in DNA-binding proteins and metabolic regulation. Our sourcing capabilities extend to validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials. Partner with CamelBio to accelerate your assay development from concept to clinic.